Molecular characterization of full-length genome of Japanese encephalitis virus genotype v isolated from Tibet, China

Ming Hua Li, Shi Hong Fu, Wei Xin Chen, Huan Yu Wang, Yu Xi Cao, Guo Dong Liang

Research output: Contribution to journalArticlepeer-review

Abstract

Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus (JEV) genotype V. Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis. Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% (G I, KV1899) to 79.7% (G III, JaGAr01), for the nucleotide sequences, and from 90.0% (G I, KV1899) to 91.8% (G III, JaGAr01) for the amino acid sequences. The open reading frame (ORF) of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides (encoded with a serine residue) were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3'-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V. Conclusions The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalBiomedical and Environmental Sciences
Volume27
Issue number4
DOIs
StatePublished - Apr 2014
Externally publishedYes

Keywords

  • Genotype V
  • Japanese encephalitis virus
  • Molecular characterization

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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