TY - JOUR
T1 - Molecular characterization of Capim and Enseada orthobunyaviruses
AU - de Souza, William Marciel
AU - Acrani, Gustavo Olszanski
AU - Romeiro, Marilia Farignoli
AU - Reis, Osvaldo
AU - Tolardo, Aline Lavado
AU - da Silva, Sandro Patroca
AU - de Almeida Medeiros, Daniele Barbosa
AU - Varela, Mariana
AU - Nunes, Márcio Roberto Teixeira
AU - Figueiredo, Luiz Tadeu Moraes
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Capim and Enseada viruses are members of the genus Orthobunyavirus isolated from mosquitoes and mammals in Brazil. Despite seroprevalence studies indicating human infections in Latin America, these viruses remain relatively unknown and unstudied. In order to better understand the genetic and evolutionary relationships among orthobunyaviruses, we sequenced the three genomic segments of Capim and Enseada orthobunyaviruses. Based on phylogenetic analysis, we demonstrated that these viruses depicted two new distinct clades, one represented by Enseada and another composed of Capim virus. In general, the genome organization and genetic traits of these viruses are similar to other orthobunyaviruses however, the open reading frame (ORF) of the putative nonstructural NSs protein of Enseada orthobunyavirus precedes the nucleocapsid ORF. Overall, our study provides details on the molecular characteristics of the prototype species of two groups within the Orthobunyavirus genus, revealing novel features into the genetic diversity and evolution of this genus.
AB - Capim and Enseada viruses are members of the genus Orthobunyavirus isolated from mosquitoes and mammals in Brazil. Despite seroprevalence studies indicating human infections in Latin America, these viruses remain relatively unknown and unstudied. In order to better understand the genetic and evolutionary relationships among orthobunyaviruses, we sequenced the three genomic segments of Capim and Enseada orthobunyaviruses. Based on phylogenetic analysis, we demonstrated that these viruses depicted two new distinct clades, one represented by Enseada and another composed of Capim virus. In general, the genome organization and genetic traits of these viruses are similar to other orthobunyaviruses however, the open reading frame (ORF) of the putative nonstructural NSs protein of Enseada orthobunyavirus precedes the nucleocapsid ORF. Overall, our study provides details on the molecular characteristics of the prototype species of two groups within the Orthobunyavirus genus, revealing novel features into the genetic diversity and evolution of this genus.
KW - Arbovirus
KW - Bunyavirus
KW - Capim orthobunyavirus
KW - Enseada orthobunyavirus
KW - Molecular characterization
KW - Orthobunyavirus
UR - http://www.scopus.com/inward/record.url?scp=84959332310&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84959332310&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2016.02.024
DO - 10.1016/j.meegid.2016.02.024
M3 - Article
C2 - 26921797
AN - SCOPUS:84959332310
SN - 1567-1348
VL - 40
SP - 47
EP - 53
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -