Molecular characterization of a hamster viscerotropic strain of yellow fever virus

Monica A. McArthur; Miguel T. Suderman; John Paul Mutebi; Shu Yuan Xiao; Alan D.T. Barrett

doi:10.1128/JVI.77.2.1462-1468.2003

Molecular characterization of a hamster viscerotropic strain of yellow fever virus

Monica A. McArthur, Miguel T. Suderman, John Paul Mutebi, Shu Yuan Xiao, Alan D.T. Barrett

Pathology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A hamster viscerotropic strain of yellow fever (YF) virus has been derived after serial passage of strain Asibi through hamsters. The parental Asibi/hamster p0 virus causes a mild and transient viremia in hamsters with no outward, clinical signs of illness. In contrast, the viscerotropic Asibi/hamster p7 virus causes a robust viremia, severe illness, and death in subadult hamsters. The genome of the hamster viscerotropic Asibi/hamster p7 virus has been sequenced and compared with the parental nonviscerotropic Asibi/hamster p0 virus identifying 14 nucleotide changes encoding only seven amino acid substitutions. The majority of these substitutions (five of seven) fall within the envelope (E) protein at positions Q27H, D28G, D155A, K323R, and K331R. These results support an important role for the E protein in determining YF virus viscerotropism.

Original language	English (US)
Pages (from-to)	1462-1468
Number of pages	7
Journal	Journal of virology
Volume	77
Issue number	2
DOIs	https://doi.org/10.1128/JVI.77.2.1462-1468.2003
State	Published - Jan 2003

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.77.2.1462-1468.2003

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abstract = "A hamster viscerotropic strain of yellow fever (YF) virus has been derived after serial passage of strain Asibi through hamsters. The parental Asibi/hamster p0 virus causes a mild and transient viremia in hamsters with no outward, clinical signs of illness. In contrast, the viscerotropic Asibi/hamster p7 virus causes a robust viremia, severe illness, and death in subadult hamsters. The genome of the hamster viscerotropic Asibi/hamster p7 virus has been sequenced and compared with the parental nonviscerotropic Asibi/hamster p0 virus identifying 14 nucleotide changes encoding only seven amino acid substitutions. The majority of these substitutions (five of seven) fall within the envelope (E) protein at positions Q27H, D28G, D155A, K323R, and K331R. These results support an important role for the E protein in determining YF virus viscerotropism.",

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AU - Xiao, Shu Yuan

AU - Barrett, Alan D.T.

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Molecular characterization of a hamster viscerotropic strain of yellow fever virus

Abstract

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