Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons

Qing Yang; Zi Zhen Wu; Xiao Li; Zhi Wang Li; Jin Bo Wei; Qi Sheng Hu

doi:10.1016/S0028-3908(02)00127-2

Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons

Qing Yang, Zi Zhen Wu, Xiao Li, Zhi Wang Li, Jin Bo Wei, Qi Sheng Hu

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The modulatory effect of oxytocin (OT) on ATP-activated currents (I_ATP) was studied in freshly isolated dorsal root ganglion (DRG) neurons of rats using whole cell clamp technique. In most of the neurons examined (50/70, 71.4%) extracellular application of OT (10^-9-10^-5 mol/L) suppressed I_ATP while in the rest (20/70, 28.6%) no modulatory effect was observed. OT shifted the ATP concentration-response curve downwards with a decrease of 39.8±4.2% in the maximal current response and with no significant change of Kd value. This OT-induced inhibition of I_ATP showed no voltage dependence, and could be blocked by [d(CH₂)₅,Tyr(Me)²,Thr⁴,Tyr-NH ₂⁹]-OVT (d(CH₂)₅-OVT) (10^-8 mol/L), a specific OT receptor antagonist. Intracellular application of H-9 (4×10^-5 mol/L, an inhibitor of protein kinase A) (n=12), BAPTA (10^-2 mol/L, a chelator of calcium ions) (n=4) could reverse the inhibitory effect of extracellular OT (10^-7 mol), while inclusion of H-7 (2×10^-5 mol/L, a protein kinase C inhibitior) (n=8) and KN-93 (10^-5 mol/L, an inhibitor of CaMKII) (n=9) in the recording pipette did not affect this effect. The results suggested that OT inhibition on ATP-activated currents was mediated by OT receptors in the membrane of DRG neurons; and this inhibitory effect involved the transduction of intracellular cAMP-PKA and Ca²⁺.

Original language	English (US)
Pages (from-to)	910-916
Number of pages	7
Journal	Neuropharmacology
Volume	43
Issue number	5
DOIs	https://doi.org/10.1016/S0028-3908(02)00127-2
State	Published - Oct 2002
Externally published	Yes

Keywords

ATP
Dorsal root ganglion
Intracellular dialysis
Oxytocin
Whole cell recording

ASJC Scopus subject areas

Pharmacology
Cellular and Molecular Neuroscience

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10.1016/S0028-3908(02)00127-2

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title = "Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons",

abstract = "The modulatory effect of oxytocin (OT) on ATP-activated currents (IATP) was studied in freshly isolated dorsal root ganglion (DRG) neurons of rats using whole cell clamp technique. In most of the neurons examined (50/70, 71.4%) extracellular application of OT (10-9-10-5 mol/L) suppressed IATP while in the rest (20/70, 28.6%) no modulatory effect was observed. OT shifted the ATP concentration-response curve downwards with a decrease of 39.8±4.2% in the maximal current response and with no significant change of Kd value. This OT-induced inhibition of IATP showed no voltage dependence, and could be blocked by [d(CH2)5,Tyr(Me)2,Thr4,Tyr-NH 29]-OVT (d(CH2)5-OVT) (10-8 mol/L), a specific OT receptor antagonist. Intracellular application of H-9 (4×10-5 mol/L, an inhibitor of protein kinase A) (n=12), BAPTA (10-2 mol/L, a chelator of calcium ions) (n=4) could reverse the inhibitory effect of extracellular OT (10-7 mol), while inclusion of H-7 (2×10-5 mol/L, a protein kinase C inhibitior) (n=8) and KN-93 (10-5 mol/L, an inhibitor of CaMKII) (n=9) in the recording pipette did not affect this effect. The results suggested that OT inhibition on ATP-activated currents was mediated by OT receptors in the membrane of DRG neurons; and this inhibitory effect involved the transduction of intracellular cAMP-PKA and Ca2+.",

keywords = "ATP, Dorsal root ganglion, Intracellular dialysis, Oxytocin, Whole cell recording",

author = "Qing Yang and Wu, {Zi Zhen} and Xiao Li and Li, {Zhi Wang} and Wei, {Jin Bo} and Hu, {Qi Sheng}",

note = "Funding Information: This work was supported by National Natural Science Foundation of China (No. 39970240)",

year = "2002",

month = oct,

doi = "10.1016/S0028-3908(02)00127-2",

language = "English (US)",

volume = "43",

pages = "910--916",

journal = "Neuropharmacology",

issn = "0028-3908",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons

AU - Yang, Qing

AU - Wu, Zi Zhen

AU - Li, Xiao

AU - Li, Zhi Wang

AU - Wei, Jin Bo

AU - Hu, Qi Sheng

N1 - Funding Information: This work was supported by National Natural Science Foundation of China (No. 39970240)

PY - 2002/10

Y1 - 2002/10

N2 - The modulatory effect of oxytocin (OT) on ATP-activated currents (IATP) was studied in freshly isolated dorsal root ganglion (DRG) neurons of rats using whole cell clamp technique. In most of the neurons examined (50/70, 71.4%) extracellular application of OT (10-9-10-5 mol/L) suppressed IATP while in the rest (20/70, 28.6%) no modulatory effect was observed. OT shifted the ATP concentration-response curve downwards with a decrease of 39.8±4.2% in the maximal current response and with no significant change of Kd value. This OT-induced inhibition of IATP showed no voltage dependence, and could be blocked by [d(CH2)5,Tyr(Me)2,Thr4,Tyr-NH 29]-OVT (d(CH2)5-OVT) (10-8 mol/L), a specific OT receptor antagonist. Intracellular application of H-9 (4×10-5 mol/L, an inhibitor of protein kinase A) (n=12), BAPTA (10-2 mol/L, a chelator of calcium ions) (n=4) could reverse the inhibitory effect of extracellular OT (10-7 mol), while inclusion of H-7 (2×10-5 mol/L, a protein kinase C inhibitior) (n=8) and KN-93 (10-5 mol/L, an inhibitor of CaMKII) (n=9) in the recording pipette did not affect this effect. The results suggested that OT inhibition on ATP-activated currents was mediated by OT receptors in the membrane of DRG neurons; and this inhibitory effect involved the transduction of intracellular cAMP-PKA and Ca2+.

AB - The modulatory effect of oxytocin (OT) on ATP-activated currents (IATP) was studied in freshly isolated dorsal root ganglion (DRG) neurons of rats using whole cell clamp technique. In most of the neurons examined (50/70, 71.4%) extracellular application of OT (10-9-10-5 mol/L) suppressed IATP while in the rest (20/70, 28.6%) no modulatory effect was observed. OT shifted the ATP concentration-response curve downwards with a decrease of 39.8±4.2% in the maximal current response and with no significant change of Kd value. This OT-induced inhibition of IATP showed no voltage dependence, and could be blocked by [d(CH2)5,Tyr(Me)2,Thr4,Tyr-NH 29]-OVT (d(CH2)5-OVT) (10-8 mol/L), a specific OT receptor antagonist. Intracellular application of H-9 (4×10-5 mol/L, an inhibitor of protein kinase A) (n=12), BAPTA (10-2 mol/L, a chelator of calcium ions) (n=4) could reverse the inhibitory effect of extracellular OT (10-7 mol), while inclusion of H-7 (2×10-5 mol/L, a protein kinase C inhibitior) (n=8) and KN-93 (10-5 mol/L, an inhibitor of CaMKII) (n=9) in the recording pipette did not affect this effect. The results suggested that OT inhibition on ATP-activated currents was mediated by OT receptors in the membrane of DRG neurons; and this inhibitory effect involved the transduction of intracellular cAMP-PKA and Ca2+.

KW - ATP

KW - Dorsal root ganglion

KW - Intracellular dialysis

KW - Oxytocin

KW - Whole cell recording

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U2 - 10.1016/S0028-3908(02)00127-2

DO - 10.1016/S0028-3908(02)00127-2

M3 - Article

C2 - 12384176

AN - SCOPUS:0036801620

SN - 0028-3908

VL - 43

SP - 910

EP - 916

JO - Neuropharmacology

JF - Neuropharmacology

IS - 5

ER -

Modulation by oxytocin of ATP-activated currents in rat dorsal root ganglion neurons

Abstract

Keywords

ASJC Scopus subject areas

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