TY - JOUR
T1 - Mitochondrial nitric oxide inhibits ATP synthesis effect of free calcium in rat heart
AU - Saavedra-Molina, Alfredo
AU - Ramírez-Emiliano, J.
AU - Clemente-Guerrero, M.
AU - Pérez-Vázquez, V.
AU - Aguilera-Aguirre, L.
AU - González-Hernández, J. C.
PY - 2003/3
Y1 - 2003/3
N2 - Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.
AB - Nitric oxide is a small potentially toxic molecule and a diatomic free radical. We report the interaction of L-arginine, oxygen and calcium with the synthesis of nitric oxide in heart mitochondria. Nitric oxide synthesis is increased in broken rat heart mitochondria compared with intact and permeabilized mitochondria. Intact mitochondria subjected to hypoxia-reoxygenation conditions accumulated nitric oxide that inhibits oxygen consumption and ATP synthesis. ATPase activity is not affected during this augment of nitric oxide. Physiological free calcium concentrations protected mitochondria from the damage caused by the accumulation of nitric oxide. Higher concentrations of the divalent cation increase the damage exerted by nitric oxide.
KW - ATP synthesis
KW - Calcium
KW - Heart
KW - Hypoxia-reoxygenation
KW - Mitochondria
KW - Nitric oxide
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U2 - 10.1007/s00726-002-0331-7
DO - 10.1007/s00726-002-0331-7
M3 - Article
C2 - 12624740
AN - SCOPUS:0037354568
SN - 0939-4451
VL - 24
SP - 95
EP - 102
JO - Amino Acids
JF - Amino Acids
IS - 1-2
ER -