Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

M. A. Endsley, M. D. Baker, E. S. Krafsur

Research output: Contribution to journalArticlepeer-review


Genomic libraries from house flies enriched for (CA)15 and (CAG)10 repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. FST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.

Original languageEnglish (US)
Pages (from-to)72-74
Number of pages3
JournalMolecular Ecology Notes
Issue number1
StatePublished - 2002
Externally publishedYes


  • Microsatellites
  • Musca autumnalis
  • Musca domestica
  • Simple sequence repeats

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • General Biochemistry, Genetics and Molecular Biology


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