Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

M. A. Endsley; M. D. Baker; E. S. Krafsur

doi:10.1046/j.1471-8286.2002.00153.x

Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

M. A. Endsley, M. D. Baker, E. S. Krafsur

Research output: Contribution to journal › Article › peer-review

Abstract

Genomic libraries from house flies enriched for (CA)₁₅ and (CAG)₁₀ repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. F_ST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.

Original language	English (US)
Pages (from-to)	72-74
Number of pages	3
Journal	Molecular Ecology Notes
Volume	2
Issue number	1
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00153.x
State	Published - 2002
Externally published	Yes

Keywords

Microsatellites
Musca autumnalis
Musca domestica
Simple sequence repeats

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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10.1046/j.1471-8286.2002.00153.x

Cite this

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abstract = "Genomic libraries from house flies enriched for (CA)15 and (CAG)10 repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. FST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.",

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AU - Endsley, M. A.

AU - Baker, M. D.

AU - Krafsur, E. S.

PY - 2002

Y1 - 2002

N2 - Genomic libraries from house flies enriched for (CA)15 and (CAG)10 repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. FST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.

AB - Genomic libraries from house flies enriched for (CA)15 and (CAG)10 repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. FST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.

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KW - Simple sequence repeats

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Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

Abstract

Keywords

ASJC Scopus subject areas

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