Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides

Per E. Andren; Mark R. Emmett; Richard M. Caprioli

doi:10.1016/1044-0305(94)87010-1

Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides

Per E. Andren, Mark R. Emmett, Richard M. Caprioli

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]⁺³ ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.

Original language	English (US)
Pages (from-to)	867-869
Number of pages	3
Journal	Journal of the American Society for Mass Spectrometry
Volume	5
Issue number	9
DOIs	https://doi.org/10.1016/1044-0305(94)87010-1
State	Published - Sep 1994
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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10.1016/1044-0305(94)87010-1

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@article{13bc577fcb06469195668886642b107d,

title = "Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides",

abstract = "The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.",

author = "Andren, {Per E.} and Emmett, {Mark R.} and Caprioli, {Richard M.}",

note = "Funding Information: Thea uthors thank NIH (grant 5 ROl GM 4378305) for partial financial support of this work. P. E. Andren thanks the Swedish Medical Research Council and the Swedish Institute for partial support.",

year = "1994",

month = sep,

doi = "10.1016/1044-0305(94)87010-1",

language = "English (US)",

volume = "5",

pages = "867--869",

journal = "Journal of the American Society for Mass Spectrometry",

issn = "1044-0305",

publisher = "Springer New York",

number = "9",

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T1 - Micro-Electrospray

T2 - Zeptomole/attomole per microliter sensitivity for peptides

AU - Andren, Per E.

AU - Emmett, Mark R.

AU - Caprioli, Richard M.

N1 - Funding Information: Thea uthors thank NIH (grant 5 ROl GM 4378305) for partial financial support of this work. P. E. Andren thanks the Swedish Medical Research Council and the Swedish Institute for partial support.

PY - 1994/9

Y1 - 1994/9

N2 - The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.

AB - The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles/μL.

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ER -

Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides

Abstract

ASJC Scopus subject areas

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