Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller; Julia Sable; Philip Goelet; Michael P. Sheetz; Virginia W. Cornish

doi:10.1002/anie.200352852

Methotrexate conjugates: A molecular in vivo protein tag

Lawrence W. Miller, Julia Sable, Philip Goelet, Michael P. Sheetz, Virginia W. Cornish

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

Original language	English (US)
Pages (from-to)	1672-1675
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	13
DOIs	https://doi.org/10.1002/anie.200352852
State	Published - Mar 19 2004
Externally published	Yes

Keywords

Bioorganic chemistry
Dihydrofolate reductase
Fluorescent probes
Methotrexate
Proteins

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200352852

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abstract = "The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).",

keywords = "Bioorganic chemistry, Dihydrofolate reductase, Fluorescent probes, Methotrexate, Proteins",

author = "Miller, {Lawrence W.} and Julia Sable and Philip Goelet and Sheetz, {Michael P.} and Cornish, {Virginia W.}",

year = "2004",

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T1 - Methotrexate conjugates

T2 - A molecular in vivo protein tag

AU - Miller, Lawrence W.

AU - Sable, Julia

AU - Goelet, Philip

AU - Sheetz, Michael P.

AU - Cornish, Virginia W.

PY - 2004/3/19

Y1 - 2004/3/19

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AB - The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

KW - Bioorganic chemistry

KW - Dihydrofolate reductase

KW - Fluorescent probes

KW - Methotrexate

KW - Proteins

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DO - 10.1002/anie.200352852

M3 - Article

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JF - Angewandte Chemie - International Edition

IS - 13

ER -

Methotrexate conjugates: A molecular in vivo protein tag

Abstract

Keywords

ASJC Scopus subject areas

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