TY - JOUR
T1 - Mediastinal adipose tissue expresses a pathogenic profile of 11 β-hydroxysteroid dehydrogenase Type 1, glucocorticoid receptor, and CD68 in patients with coronary artery disease
AU - Atalar, Fatmahan
AU - Gormez, Selcuk
AU - Caynak, Baris
AU - Akan, Gokce
AU - Tanriverdi, Gamze
AU - Bilgic-Gazioglu, Sema
AU - Gunay, Demet
AU - Duran, Cihan
AU - Akpinar, Belhhan
AU - Ozbek, Ugur
AU - Buyukdevrim, Ahmet Sevim
AU - Yazici, Zeliha
N1 - Funding Information:
Funding: This study was funded by Turkish Diabetes Foundation and Sanovel Pharmaceuticals Company.
PY - 2013/5
Y1 - 2013/5
N2 - Objective: Cardiac visceral fat is accepted to be a new marker for cardiometabolic risk due to its association with increased cardiovascular risk factors. This study aimed to compare the expression of 11 beta hydroxysteroid dehydrogenases (11β-HSD)-1, glucocorticoid receptor (GCR), and CD68 in mediastinal and subcutaneous adipose tissues (MAT, and SAT, respectively) and to assess their possible relationships with the development of coronary artery disease (CAD). Methods and results: Expression of 11β-HSD-1, GCR, and CD68 mRNA levels were measured by quantitative real-time polymerase chain reaction in MAT and SAT tissues of 37 patients undergoing coronary artery bypass grafting due to CAD (CAD group) and 19 non-CAD patients (controls) undergoing heart valve surgery. 11β-HSD-1 in MAT and SAT and GCR expression in MAT and SAT were found to be significantly increased in CAD group when compared with controls (P<.05, respectively). In CAD group, 11β-HSD-1 mRNA levels were found to be significantly higher in MAT compared to SAT (P<.05). CD68 mRNA levels were significantly higher in MAT of CAD group compared to controls (P<.05). Immunohistochemical analyses demonstrated the presence of CD68+ cells and increased 11β-HSD-1 expression in MAT of CAD group compared to SAT. Conclusion: The present study demonstrate that the mediastinal fat exhibits a pathogenic mRNA profile of 11β-HSD-1, GCR, and CD68. The identification of 11β-HSD-1 expression within the mediastinal fat, along with increased GCR expressions and the presence of CD68+ cells highlight that MAT potentially contributes to the pathogenesis of CAD.
AB - Objective: Cardiac visceral fat is accepted to be a new marker for cardiometabolic risk due to its association with increased cardiovascular risk factors. This study aimed to compare the expression of 11 beta hydroxysteroid dehydrogenases (11β-HSD)-1, glucocorticoid receptor (GCR), and CD68 in mediastinal and subcutaneous adipose tissues (MAT, and SAT, respectively) and to assess their possible relationships with the development of coronary artery disease (CAD). Methods and results: Expression of 11β-HSD-1, GCR, and CD68 mRNA levels were measured by quantitative real-time polymerase chain reaction in MAT and SAT tissues of 37 patients undergoing coronary artery bypass grafting due to CAD (CAD group) and 19 non-CAD patients (controls) undergoing heart valve surgery. 11β-HSD-1 in MAT and SAT and GCR expression in MAT and SAT were found to be significantly increased in CAD group when compared with controls (P<.05, respectively). In CAD group, 11β-HSD-1 mRNA levels were found to be significantly higher in MAT compared to SAT (P<.05). CD68 mRNA levels were significantly higher in MAT of CAD group compared to controls (P<.05). Immunohistochemical analyses demonstrated the presence of CD68+ cells and increased 11β-HSD-1 expression in MAT of CAD group compared to SAT. Conclusion: The present study demonstrate that the mediastinal fat exhibits a pathogenic mRNA profile of 11β-HSD-1, GCR, and CD68. The identification of 11β-HSD-1 expression within the mediastinal fat, along with increased GCR expressions and the presence of CD68+ cells highlight that MAT potentially contributes to the pathogenesis of CAD.
KW - 11 beta hydroxysteroid dehydrogenase
KW - Coronary artery disease
KW - Glucocorticoid receptor
KW - Mediastinal adipose tissue
KW - Subcutaneous adipose tissue
UR - http://www.scopus.com/inward/record.url?scp=84877690893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877690893&partnerID=8YFLogxK
U2 - 10.1016/j.carpath.2012.07.006
DO - 10.1016/j.carpath.2012.07.006
M3 - Article
C2 - 22955009
AN - SCOPUS:84877690893
SN - 1054-8807
VL - 22
SP - 183
EP - 188
JO - Cardiovascular Pathology
JF - Cardiovascular Pathology
IS - 3
ER -