TY - JOUR
T1 - Mechanism of action of glucocorticoid-induced immunoglobulin production
T2 - II. Requirement for fetal calf serum
AU - Sierakowski, S.
AU - Goodwin, J. S.
PY - 1988
Y1 - 1988
N2 - Corticosteroids have been reported to stimulate immunoglobulin (Ig) production by otherwise unstimulated peripheral blood mononuclear cells. In this paper we confirm that observation but report the necessity for an additional minor antigenic stimulus. Lymphocytes cultured with HB101, a complete medium not requiring serum supplement, did not increase Ig production after addition of hydrocortisone, but did if small concentrations (1-10%) of fetal calf serum were added. Various antigens such as candida, tetanus, and albumin of different sources could substitute for fetal calf sera in allowing corticosteroids to stimulate Ig production in resting lymphocytes. We conclude that corticosteroids do not stimulate Ig production by lymphocytes de novo; rather, corticosteroids greatly enhance the Ig production stimulated by the foreign antigens present in fetal calf sera.
AB - Corticosteroids have been reported to stimulate immunoglobulin (Ig) production by otherwise unstimulated peripheral blood mononuclear cells. In this paper we confirm that observation but report the necessity for an additional minor antigenic stimulus. Lymphocytes cultured with HB101, a complete medium not requiring serum supplement, did not increase Ig production after addition of hydrocortisone, but did if small concentrations (1-10%) of fetal calf serum were added. Various antigens such as candida, tetanus, and albumin of different sources could substitute for fetal calf sera in allowing corticosteroids to stimulate Ig production in resting lymphocytes. We conclude that corticosteroids do not stimulate Ig production by lymphocytes de novo; rather, corticosteroids greatly enhance the Ig production stimulated by the foreign antigens present in fetal calf sera.
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M3 - Article
C2 - 3349647
AN - SCOPUS:0023864161
SN - 0009-9104
VL - 71
SP - 198
EP - 201
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 1
ER -