TY - JOUR
T1 - Measuring Exercise Capacity and Physical Function in Adult and Older Mice
AU - Graber, Ted G.
AU - Maroto, Rosario
AU - Fry, Christopher S.
AU - Brightwell, Camille R.
AU - Rasmussen, Blake B.
N1 - Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: [email protected].
PY - 2021/5/1
Y1 - 2021/5/1
N2 - The inability of older adults to maintain independence is a consequence of sarcopenia and frailty. In order to identify the molecular mechanisms responsible for decreased physical function, it will be critical to utilize a small animal model. The main purpose of this study was to develop a composite Comprehensive Functional Assessment Battery (CFAB) of well-validated tests to determine physical function and exercise capacity in 3 age groups of male C57BL/6 mice (6 months old, n = 29; 24 months old, n = 24; 28+ months old, n = 28). To measure physical function in mice, we used rotarod (overall motor function), grip meter (forelimb strength), treadmill (endurance), inverted cling (strength/endurance), voluntary wheel running (volitional exercise and activity rate), and muscle performance with in vivo contractile physiology (dorsiflexor torque). We hypothesized that CFAB would be a valid means to assess the physical function of a given mouse across the life span. In addition, we proposed that CFAB could be used to determine relationships between different parameters associated with sarcopenia. We found that there was an overall age-related significant decline (p <. 05) in all measurements, and the CFAB score demonstrated that some individual mice (the upper quartile) retained the functional capacity of average mice 1 cohort younger. We conclude that the CFAB is a powerful, repeatable, and noninvasive tool to assess and compare physical function and assess complex motor task ability in mice, which will enable researchers to easily track performance at the individual mouse level.
AB - The inability of older adults to maintain independence is a consequence of sarcopenia and frailty. In order to identify the molecular mechanisms responsible for decreased physical function, it will be critical to utilize a small animal model. The main purpose of this study was to develop a composite Comprehensive Functional Assessment Battery (CFAB) of well-validated tests to determine physical function and exercise capacity in 3 age groups of male C57BL/6 mice (6 months old, n = 29; 24 months old, n = 24; 28+ months old, n = 28). To measure physical function in mice, we used rotarod (overall motor function), grip meter (forelimb strength), treadmill (endurance), inverted cling (strength/endurance), voluntary wheel running (volitional exercise and activity rate), and muscle performance with in vivo contractile physiology (dorsiflexor torque). We hypothesized that CFAB would be a valid means to assess the physical function of a given mouse across the life span. In addition, we proposed that CFAB could be used to determine relationships between different parameters associated with sarcopenia. We found that there was an overall age-related significant decline (p <. 05) in all measurements, and the CFAB score demonstrated that some individual mice (the upper quartile) retained the functional capacity of average mice 1 cohort younger. We conclude that the CFAB is a powerful, repeatable, and noninvasive tool to assess and compare physical function and assess complex motor task ability in mice, which will enable researchers to easily track performance at the individual mouse level.
KW - Frailty
KW - Function
KW - Mouse models
KW - Muscle
KW - Neuromuscular decline
KW - Sarcopenia
UR - http://www.scopus.com/inward/record.url?scp=85105029338&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85105029338&partnerID=8YFLogxK
U2 - 10.1093/gerona/glaa205
DO - 10.1093/gerona/glaa205
M3 - Article
C2 - 32822475
AN - SCOPUS:85105029338
SN - 1079-5006
VL - 76
SP - 819
EP - 824
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 5
ER -