Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata

Reinhard Predel; William K. Russell; Shane E. Tichy; David H. Russell; Ronald J. Nachman

doi:10.1016/j.peptides.2003.06.009

Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata

Reinhard Predel, William K. Russell, Shane E. Tichy, David H. Russell, Ronald J. Nachman

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Neuropeptides of the capa-gene are typical of the abdominal neurosecretory system of insects. In this study, we investigated these peptides in two widely distributed and large pest flies, namely Musca domestica and Neobellieria bullata. Using a combination of MALDI-TOF and ESI-QTOF mass spectrometry, periviscerokinins and a pyrokinin were analyzed from single perisympathetic organ preparations. The species-specific peptide sequences differ remarkably between the related dipteran species. These differences could make it possible to develop peptide-analogs with group- or species-specific efficacy.

Original language	English (US)
Pages (from-to)	1487-1491
Number of pages	5
Journal	Peptides
Volume	24
Issue number	10
DOIs	https://doi.org/10.1016/j.peptides.2003.06.009
State	Published - Oct 2003
Externally published	Yes

Keywords

CAP
Insect neuropeptide
Mass spectrometry
Musca domestica
Neobellieria bullata
Perisympathetic organs
Periviscerokinin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/j.peptides.2003.06.009

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@article{4286ebb7c05a4ac3997372dba1ff851d,

title = "Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata",

abstract = "Neuropeptides of the capa-gene are typical of the abdominal neurosecretory system of insects. In this study, we investigated these peptides in two widely distributed and large pest flies, namely Musca domestica and Neobellieria bullata. Using a combination of MALDI-TOF and ESI-QTOF mass spectrometry, periviscerokinins and a pyrokinin were analyzed from single perisympathetic organ preparations. The species-specific peptide sequences differ remarkably between the related dipteran species. These differences could make it possible to develop peptide-analogs with group- or species-specific efficacy.",

keywords = "CAP, Insect neuropeptide, Mass spectrometry, Musca domestica, Neobellieria bullata, Perisympathetic organs, Periviscerokinin",

author = "Reinhard Predel and Russell, {William K.} and Tichy, {Shane E.} and Russell, {David H.} and Nachman, {Ronald J.}",

note = "Funding Information: This study was supported with a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1), a Binational Agricultural Research and Development Grant (BARD #3356-02) (R.J.N.) and a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (R.J.N.). In addition, we acknowledge the capable technical assistance of Allison Strey and David Rigolout of the Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center. ",

year = "2003",

month = oct,

doi = "10.1016/j.peptides.2003.06.009",

language = "English (US)",

volume = "24",

pages = "1487--1491",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "10",

}

TY - JOUR

T1 - Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata

AU - Predel, Reinhard

AU - Russell, William K.

AU - Tichy, Shane E.

AU - Russell, David H.

AU - Nachman, Ronald J.

N1 - Funding Information: This study was supported with a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1), a Binational Agricultural Research and Development Grant (BARD #3356-02) (R.J.N.) and a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (R.J.N.). In addition, we acknowledge the capable technical assistance of Allison Strey and David Rigolout of the Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center.

PY - 2003/10

Y1 - 2003/10

N2 - Neuropeptides of the capa-gene are typical of the abdominal neurosecretory system of insects. In this study, we investigated these peptides in two widely distributed and large pest flies, namely Musca domestica and Neobellieria bullata. Using a combination of MALDI-TOF and ESI-QTOF mass spectrometry, periviscerokinins and a pyrokinin were analyzed from single perisympathetic organ preparations. The species-specific peptide sequences differ remarkably between the related dipteran species. These differences could make it possible to develop peptide-analogs with group- or species-specific efficacy.

AB - Neuropeptides of the capa-gene are typical of the abdominal neurosecretory system of insects. In this study, we investigated these peptides in two widely distributed and large pest flies, namely Musca domestica and Neobellieria bullata. Using a combination of MALDI-TOF and ESI-QTOF mass spectrometry, periviscerokinins and a pyrokinin were analyzed from single perisympathetic organ preparations. The species-specific peptide sequences differ remarkably between the related dipteran species. These differences could make it possible to develop peptide-analogs with group- or species-specific efficacy.

KW - CAP

KW - Insect neuropeptide

KW - Mass spectrometry

KW - Musca domestica

KW - Neobellieria bullata

KW - Perisympathetic organs

KW - Periviscerokinin

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DO - 10.1016/j.peptides.2003.06.009

M3 - Article

C2 - 14706527

AN - SCOPUS:0346099139

SN - 0196-9781

VL - 24

SP - 1487

EP - 1491

JO - Peptides

JF - Peptides

IS - 10

ER -

Mass spectrometric analysis of putative capa-gene products in Musca domestica and Neobellieria bullata

Abstract

Keywords

ASJC Scopus subject areas

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