Low-ionic-strength saline solution-antiglobulin test (LISS-AGT)

Lee Ann Walker

    Research output: Contribution to journalReview articlepeer-review


    The use of low-ionic-strength saline (LISS) solution as an enhancement for antibody screening and crossmatching was first described by Löw and Messeter in 1974. This method allowed for a reduced incubation time while maintaining adequate specificity and sensitivity of the antiglobulin test (AGT). Since then, the LISS-AGT tube method has been widely used in antibody detection and identification, as well as compatibility testing. As initially described, the method used red blood cells suspended in LISS. Modifications of the method led to development of the commercially prepared LISS additive solutions in use today. The LISS-AGT can be used effectively to detect alloantibodies of all major blood groups in antibody detection, antibody identification, and crossmatching procedures.

    Original languageEnglish (US)
    Pages (from-to)57-60
    Number of pages4
    Issue number2
    StatePublished - 2018


    • Antibody detection
    • Antiglobulin test
    • Enhancement
    • LISS
    • LISS-AGT
    • Low-ionic-strength saline

    ASJC Scopus subject areas

    • Hematology
    • Immunology and Allergy


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