Long polar fimbriae and tissue tropism in Escherichia coli O157:H7

Robert Fitzhenry; Sivan Dahan; Alfredo G. Torres; Yuwen Chong; Robert Heuschkel; Simon H. Murch; Mike Thomson; James B. Kaper; Gad Frankel; Alan D. Phillips

doi:10.1016/j.micinf.2006.02.012

Long polar fimbriae and tissue tropism in Escherichia coli O157:H7

Robert Fitzhenry, Sivan Dahan, Alfredo G. Torres, Yuwen Chong, Robert Heuschkel, Simon H. Murch, Mike Thomson, James B. Kaper, Gad Frankel, Alan D. Phillips

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

In vitro organ culture has demonstrated the human intestinal tropism of enterohaemorrhagic Escherichia coli O157:H7 for follicle associated epithelium overlying Peyer's patches of the terminal ileum. Long polar (LP) fimbriae are considered to mediate the attachment of Salmonella enterica serovar Typhimurium to Peyer's patch epithelium and, as homologous genes have been identified in O157:H7, we hypothesised that LP fimbriae in O157:H7 may perform the same function. However, mutation of LP fimbriae in O157:H7 strain 85/170 resulted in the novel phenotype of proximal and distal small intestinal colonisation with attaching/effacing lesion formation, while retaining adhesion to follicle associated epithelium. Application of whole genome DNA array technology did not identify changes in known fimbrial genes that could explain the change in tropism, but highlighted several genes that require further investigation. LP fimbrial genes are the first genes to be identified outside the locus of enterocyte effacement pathogenicity island that influence O157:H7 human intestinal tissue tropism.

Original language	English (US)
Pages (from-to)	1741-1749
Number of pages	9
Journal	Microbes and Infection
Volume	8
Issue number	7
DOIs	https://doi.org/10.1016/j.micinf.2006.02.012
State	Published - Jun 2006

Keywords

Fimbriae
Intestine
O157:H7
Organ culture
Tropism

ASJC Scopus subject areas

Microbiology
Immunology
Infectious Diseases

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10.1016/j.micinf.2006.02.012

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@article{906896d83fc94ba594b55aed0416568e,

title = "Long polar fimbriae and tissue tropism in Escherichia coli O157:H7",

abstract = "In vitro organ culture has demonstrated the human intestinal tropism of enterohaemorrhagic Escherichia coli O157:H7 for follicle associated epithelium overlying Peyer's patches of the terminal ileum. Long polar (LP) fimbriae are considered to mediate the attachment of Salmonella enterica serovar Typhimurium to Peyer's patch epithelium and, as homologous genes have been identified in O157:H7, we hypothesised that LP fimbriae in O157:H7 may perform the same function. However, mutation of LP fimbriae in O157:H7 strain 85/170 resulted in the novel phenotype of proximal and distal small intestinal colonisation with attaching/effacing lesion formation, while retaining adhesion to follicle associated epithelium. Application of whole genome DNA array technology did not identify changes in known fimbrial genes that could explain the change in tropism, but highlighted several genes that require further investigation. LP fimbrial genes are the first genes to be identified outside the locus of enterocyte effacement pathogenicity island that influence O157:H7 human intestinal tissue tropism.",

keywords = "Fimbriae, Intestine, O157:H7, Organ culture, Tropism",

author = "Robert Fitzhenry and Sivan Dahan and Torres, {Alfredo G.} and Yuwen Chong and Robert Heuschkel and Murch, {Simon H.} and Mike Thomson and Kaper, {James B.} and Gad Frankel and Phillips, {Alan D.}",

note = "Funding Information: We thank C. Toma, Department of Bacteriology, University of the Ryukyus, Japan, and Soman Abraham, Duke University Medical Center for the type I fimbrial specific antibodies. The work was funded by a BBSRC grant to ADP and GF. JBK was supported by NIH grant no. DK58957. AGT was supported by institutional funds from the UTMB John Sealy Memorial Endowment Fund for Biomedical Research and the Gastro Intestinal Research Interdisciplinary Program. ",

year = "2006",

month = jun,

doi = "10.1016/j.micinf.2006.02.012",

language = "English (US)",

volume = "8",

pages = "1741--1749",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson SAS",

number = "7",

}

TY - JOUR

T1 - Long polar fimbriae and tissue tropism in Escherichia coli O157:H7

AU - Fitzhenry, Robert

AU - Dahan, Sivan

AU - Torres, Alfredo G.

AU - Chong, Yuwen

AU - Heuschkel, Robert

AU - Murch, Simon H.

AU - Thomson, Mike

AU - Kaper, James B.

AU - Frankel, Gad

AU - Phillips, Alan D.

N1 - Funding Information: We thank C. Toma, Department of Bacteriology, University of the Ryukyus, Japan, and Soman Abraham, Duke University Medical Center for the type I fimbrial specific antibodies. The work was funded by a BBSRC grant to ADP and GF. JBK was supported by NIH grant no. DK58957. AGT was supported by institutional funds from the UTMB John Sealy Memorial Endowment Fund for Biomedical Research and the Gastro Intestinal Research Interdisciplinary Program.

PY - 2006/6

Y1 - 2006/6

N2 - In vitro organ culture has demonstrated the human intestinal tropism of enterohaemorrhagic Escherichia coli O157:H7 for follicle associated epithelium overlying Peyer's patches of the terminal ileum. Long polar (LP) fimbriae are considered to mediate the attachment of Salmonella enterica serovar Typhimurium to Peyer's patch epithelium and, as homologous genes have been identified in O157:H7, we hypothesised that LP fimbriae in O157:H7 may perform the same function. However, mutation of LP fimbriae in O157:H7 strain 85/170 resulted in the novel phenotype of proximal and distal small intestinal colonisation with attaching/effacing lesion formation, while retaining adhesion to follicle associated epithelium. Application of whole genome DNA array technology did not identify changes in known fimbrial genes that could explain the change in tropism, but highlighted several genes that require further investigation. LP fimbrial genes are the first genes to be identified outside the locus of enterocyte effacement pathogenicity island that influence O157:H7 human intestinal tissue tropism.

AB - In vitro organ culture has demonstrated the human intestinal tropism of enterohaemorrhagic Escherichia coli O157:H7 for follicle associated epithelium overlying Peyer's patches of the terminal ileum. Long polar (LP) fimbriae are considered to mediate the attachment of Salmonella enterica serovar Typhimurium to Peyer's patch epithelium and, as homologous genes have been identified in O157:H7, we hypothesised that LP fimbriae in O157:H7 may perform the same function. However, mutation of LP fimbriae in O157:H7 strain 85/170 resulted in the novel phenotype of proximal and distal small intestinal colonisation with attaching/effacing lesion formation, while retaining adhesion to follicle associated epithelium. Application of whole genome DNA array technology did not identify changes in known fimbrial genes that could explain the change in tropism, but highlighted several genes that require further investigation. LP fimbrial genes are the first genes to be identified outside the locus of enterocyte effacement pathogenicity island that influence O157:H7 human intestinal tissue tropism.

KW - Fimbriae

KW - Intestine

KW - O157:H7

KW - Organ culture

KW - Tropism

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JO - Microbes and Infection

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ER -

Long polar fimbriae and tissue tropism in Escherichia coli O157:H7

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