Location of the enterotoxin gene from Salmonella typhimurium and characterization of the gene products

P. Chary; R. Prasad; A. K. Chopra; J. W. Peterson

doi:10.1111/j.1574-6968.1993.tb06366.x

Location of the enterotoxin gene from Salmonella typhimurium and characterization of the gene products

P. Chary, R. Prasad, A. K. Chopra, J. W. Peterson

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The enterotoxin gene (stn) in Salmonella typhimurium (Q1 strain) was confined to an 800 bp ClaI-EcoRI genomic DNA fragment (pCE3) that coded for two polypeptides (25 and 12 kDa) under the control of the T7 RNA polymerase/promoter system. The appearance of the 25 kDa protein corresponded to the enterotoxic activity, as determined by elongation of Chinese hamster ovary (CHO) cells, fluid accumulation in rabbit intestinal loops, and altered vascular permeability in rabbit skin. The stn gene products (STN) caused an elevation of intracellular cAMP in CHO cells. These values were at control levels in stn mutants devoid of enterotoxicity, and the 25-kDa protein concurrently disappeared. The biological activity of the heat-labile enterotoxin was blocked by G_M1 ganglioside and neutralized by affinity-purified antibodies made against cholera toxin. The 12 kDa protein however was not correlated with an enterotoxic response.

Original language	English (US)
Pages (from-to)	87-92
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	111
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06366.x
State	Published - Jul 15 1993

Keywords

STN
Salmonella typhimurium
T7 RNA polymerase/promoter
cAMP
stn Gene
stn Mutant

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1993.tb06366.x

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title = "Location of the enterotoxin gene from Salmonella typhimurium and characterization of the gene products",

abstract = "The enterotoxin gene (stn) in Salmonella typhimurium (Q1 strain) was confined to an 800 bp ClaI-EcoRI genomic DNA fragment (pCE3) that coded for two polypeptides (25 and 12 kDa) under the control of the T7 RNA polymerase/promoter system. The appearance of the 25 kDa protein corresponded to the enterotoxic activity, as determined by elongation of Chinese hamster ovary (CHO) cells, fluid accumulation in rabbit intestinal loops, and altered vascular permeability in rabbit skin. The stn gene products (STN) caused an elevation of intracellular cAMP in CHO cells. These values were at control levels in stn mutants devoid of enterotoxicity, and the 25-kDa protein concurrently disappeared. The biological activity of the heat-labile enterotoxin was blocked by GM1 ganglioside and neutralized by affinity-purified antibodies made against cholera toxin. The 12 kDa protein however was not correlated with an enterotoxic response.",

keywords = "STN, Salmonella typhimurium, T7 RNA polymerase/promoter, cAMP, stn Gene, stn Mutant",

author = "P. Chary and R. Prasad and Chopra, {A. K.} and Peterson, {J. W.}",

note = "Funding Information: We would like to thank Juan Cantu for his technicaal ssistancien performingth e cAMP as-saysw ith CHO cells. This studyw as supportebdy researchg rantR01 18401-09fr om NIH.P.C. was the recipiento f a postdoctorafle llowshipf rom the J.W. McLaughlinF ellowshipF und.",

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AU - Prasad, R.

AU - Chopra, A. K.

AU - Peterson, J. W.

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PY - 1993/7/15

Y1 - 1993/7/15

N2 - The enterotoxin gene (stn) in Salmonella typhimurium (Q1 strain) was confined to an 800 bp ClaI-EcoRI genomic DNA fragment (pCE3) that coded for two polypeptides (25 and 12 kDa) under the control of the T7 RNA polymerase/promoter system. The appearance of the 25 kDa protein corresponded to the enterotoxic activity, as determined by elongation of Chinese hamster ovary (CHO) cells, fluid accumulation in rabbit intestinal loops, and altered vascular permeability in rabbit skin. The stn gene products (STN) caused an elevation of intracellular cAMP in CHO cells. These values were at control levels in stn mutants devoid of enterotoxicity, and the 25-kDa protein concurrently disappeared. The biological activity of the heat-labile enterotoxin was blocked by GM1 ganglioside and neutralized by affinity-purified antibodies made against cholera toxin. The 12 kDa protein however was not correlated with an enterotoxic response.

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KW - cAMP

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KW - stn Mutant

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Location of the enterotoxin gene from Salmonella typhimurium and characterization of the gene products

Abstract

Keywords

ASJC Scopus subject areas

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