Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher, Meike Heidbreder, Sebastian Van De Linde, Michael P. Sheetz, Mike Heilemann, Virginia W. Cornish, Markus Sauer

Research output: Contribution to journalArticlepeer-review

259 Scopus citations


The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.

Original languageEnglish (US)
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Issue number9
StatePublished - Sep 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Live-cell super-resolution imaging with trimethoprim conjugates'. Together they form a unique fingerprint.

Cite this