Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon

Maya Williams; Sandra V. Mayer; William L. Johnson; Rubing Chen; Evgeniya Volkova; Stalin Vilcarromero; Steven G. Widen; Thomas G. Wood; Luis Suarez-Ognio; Kanya C. Long; Kathryn A. Hanley; Amy C. Morrison; Nikos Vasilakis; Eric S. Halsey

doi:10.4269/ajtmh.13-0600

Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon

Maya Williams, Sandra V. Mayer, William L. Johnson, Rubing Chen, Evgeniya Volkova, Stalin Vilcarromero, Steven G. Widen, Thomas G. Wood, Luis Suarez-Ognio, Kanya C. Long, Kathryn A. Hanley, Amy C. Morrison, Nikos Vasilakis, Eric S. Halsey

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30 Scopus citations

Abstract

During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.

Original language	English (US)
Pages (from-to)	611-620
Number of pages	10
Journal	American Journal of Tropical Medicine and Hygiene
Volume	91
Issue number	3
DOIs	https://doi.org/10.4269/ajtmh.13-0600
State	Published - Sep 1 2014

ASJC Scopus subject areas

Parasitology
Infectious Diseases
Virology

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Williams, M., Mayer, S. V., Johnson, W. L., Chen, R., Volkova, E., Vilcarromero, S., Widen, S. G., Wood, T. G., Suarez-Ognio, L., Long, K. C., Hanley, K. A., Morrison, A. C., Vasilakis, N., & Halsey, E. S. (2014). Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon. American Journal of Tropical Medicine and Hygiene, 91(3), 611-620. https://doi.org/10.4269/ajtmh.13-0600

Williams, M, Mayer, SV, Johnson, WL, Chen, R, Volkova, E, Vilcarromero, S, Widen, SG, Wood, TG, Suarez-Ognio, L, Long, KC, Hanley, KA, Morrison, AC, Vasilakis, N & Halsey, ES 2014, 'Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon', American Journal of Tropical Medicine and Hygiene, vol. 91, no. 3, pp. 611-620. https://doi.org/10.4269/ajtmh.13-0600

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title = "Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon",

abstract = "During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.",

author = "Maya Williams and Mayer, {Sandra V.} and Johnson, {William L.} and Rubing Chen and Evgeniya Volkova and Stalin Vilcarromero and Widen, {Steven G.} and Wood, {Thomas G.} and Luis Suarez-Ognio and Long, {Kanya C.} and Hanley, {Kathryn A.} and Morrison, {Amy C.} and Nikos Vasilakis and Halsey, {Eric S.}",

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AU - Mayer, Sandra V.

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AU - Volkova, Evgeniya

AU - Vilcarromero, Stalin

AU - Widen, Steven G.

AU - Wood, Thomas G.

AU - Suarez-Ognio, Luis

AU - Long, Kanya C.

AU - Hanley, Kathryn A.

AU - Morrison, Amy C.

AU - Vasilakis, Nikos

AU - Halsey, Eric S.

PY - 2014/9/1

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N2 - During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.

AB - During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.

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Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon

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