Las células dendríticas plasmacitoides evocan la respuesta efectora de CTL Salmonella-específicos

Carolina del Carmen Murúa-López; María González-Orozco; Héctor Samuel López-Moreno

doi:10.7705/biomedica.v39i3.4349

Las células dendríticas plasmacitoides evocan la respuesta efectora de CTL Salmonella-específicos

Translated title of the contribution: Plasmacytoid dendritic cells evoke the Salmonella-specific CTL effector response

Carolina del Carmen Murúa-López, María González-Orozco, Héctor Samuel López-Moreno

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: The immunological role of Plasmacytoid Dendritic Cells (pDC) in bacterial infections such as Salmonella, is poorly known. Therefore, we analysed the effector function of pDC to present S. Typhimurium antigens to cytotoxic T lymphocytes (CTL). Objective: To analyze the cytotoxic T Lymphocytes Salmonella-specific response evoked by Plasmacytoid Dendritic Cells (pDC). Material and methods: pDC CFSE-stained and pulsed withOmpC73 Salmonella Kb-restricted epitopes or S. Typhimurium infected, were used as target in cytotoxicity assays. Results: Specific lysis was statically significant in pDC+OmpC73 for all E:T ratios (p ≤ 0.05); for pDC+S. Typhimurium statistical significance was only observed at 1:100 ratio (p ≤ 0.05) using OmpC73. Conclusion: pDC can evoke cytotoxic T lymphocytes responses during S. Typhimurium infection.

Translated title of the contribution	Plasmacytoid dendritic cells evoke the Salmonella-specific CTL effector response
Original language	Spanish
Pages (from-to)	172-181
Number of pages	10
Journal	Biomedica
Volume	39
DOIs	https://doi.org/10.7705/biomedica.v39i3.4349
State	Published - 2019
Externally published	Yes

Keywords

Cytotoxic
Cytotoxicity
Dendritic cells
Epitopes
Immunologic
Salmonella
T-lymphocytes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.7705/biomedica.v39i3.4349

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@article{1d5ef7abfcb4433482ee999f6685ef38,

title = "Las c{\'e}lulas dendr{\'i}ticas plasmacitoides evocan la respuesta efectora de CTL Salmonella-espec{\'i}ficos",

abstract = "Introduction: The immunological role of Plasmacytoid Dendritic Cells (pDC) in bacterial infections such as Salmonella, is poorly known. Therefore, we analysed the effector function of pDC to present S. Typhimurium antigens to cytotoxic T lymphocytes (CTL). Objective: To analyze the cytotoxic T Lymphocytes Salmonella-specific response evoked by Plasmacytoid Dendritic Cells (pDC). Material and methods: pDC CFSE-stained and pulsed withOmpC73 Salmonella Kb-restricted epitopes or S. Typhimurium infected, were used as target in cytotoxicity assays. Results: Specific lysis was statically significant in pDC+OmpC73 for all E:T ratios (p ≤ 0.05); for pDC+S. Typhimurium statistical significance was only observed at 1:100 ratio (p ≤ 0.05) using OmpC73. Conclusion: pDC can evoke cytotoxic T lymphocytes responses during S. Typhimurium infection.",

keywords = "Cytotoxic, Cytotoxicity, Dendritic cells, Epitopes, Immunologic, Salmonella, T-lymphocytes",

author = "Mur{\'u}a-L{\'o}pez, {Carolina del Carmen} and Mar{\'i}a Gonz{\'a}lez-Orozco and L{\'o}pez-Moreno, {H{\'e}ctor Samuel}",

note = "Publisher Copyright: {\textcopyright} 2019 Instituto Nacional de Salud.",

year = "2019",

doi = "10.7705/biomedica.v39i3.4349",

language = "Spanish",

volume = "39",

pages = "172--181",

journal = "Biomedica",

issn = "0120-4157",

publisher = "Instituto Nacional de Salud",

}

TY - JOUR

T1 - Las células dendríticas plasmacitoides evocan la respuesta efectora de CTL Salmonella-específicos

AU - Murúa-López, Carolina del Carmen

AU - González-Orozco, María

AU - López-Moreno, Héctor Samuel

PY - 2019

Y1 - 2019

N2 - Introduction: The immunological role of Plasmacytoid Dendritic Cells (pDC) in bacterial infections such as Salmonella, is poorly known. Therefore, we analysed the effector function of pDC to present S. Typhimurium antigens to cytotoxic T lymphocytes (CTL). Objective: To analyze the cytotoxic T Lymphocytes Salmonella-specific response evoked by Plasmacytoid Dendritic Cells (pDC). Material and methods: pDC CFSE-stained and pulsed withOmpC73 Salmonella Kb-restricted epitopes or S. Typhimurium infected, were used as target in cytotoxicity assays. Results: Specific lysis was statically significant in pDC+OmpC73 for all E:T ratios (p ≤ 0.05); for pDC+S. Typhimurium statistical significance was only observed at 1:100 ratio (p ≤ 0.05) using OmpC73. Conclusion: pDC can evoke cytotoxic T lymphocytes responses during S. Typhimurium infection.

AB - Introduction: The immunological role of Plasmacytoid Dendritic Cells (pDC) in bacterial infections such as Salmonella, is poorly known. Therefore, we analysed the effector function of pDC to present S. Typhimurium antigens to cytotoxic T lymphocytes (CTL). Objective: To analyze the cytotoxic T Lymphocytes Salmonella-specific response evoked by Plasmacytoid Dendritic Cells (pDC). Material and methods: pDC CFSE-stained and pulsed withOmpC73 Salmonella Kb-restricted epitopes or S. Typhimurium infected, were used as target in cytotoxicity assays. Results: Specific lysis was statically significant in pDC+OmpC73 for all E:T ratios (p ≤ 0.05); for pDC+S. Typhimurium statistical significance was only observed at 1:100 ratio (p ≤ 0.05) using OmpC73. Conclusion: pDC can evoke cytotoxic T lymphocytes responses during S. Typhimurium infection.

KW - Cytotoxic

KW - Cytotoxicity

KW - Dendritic cells

KW - Epitopes

KW - Immunologic

KW - Salmonella

KW - T-lymphocytes

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DO - 10.7705/biomedica.v39i3.4349

M3 - Article

C2 - 31529843

AN - SCOPUS:85067130909

SN - 0120-4157

VL - 39

SP - 172

EP - 181

JO - Biomedica

JF - Biomedica

ER -

Las células dendríticas plasmacitoides evocan la respuesta efectora de CTL Salmonella-específicos

Abstract

Keywords

ASJC Scopus subject areas

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