Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats

Janusz T. Paweska; Petrus Jansen Van Vuren; Karla A. Fenton; Kerry Graves; Antoinette A. Grobbelaar; Naazneen Moolla; Patricia Leman; Jacqueline Weyer; Nadia Storm; Stewart D. McCulloch; Terence P. Scott; Wanda Markotter; Lieza Odendaal; Sarah J. Clift; Thomas W. Geisbert; Martin J. Hale; Alan Kemp

doi:10.1093/infdis/jiv132

Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats

Janusz T. Paweska, Petrus Jansen Van Vuren, Karla A. Fenton, Kerry Graves, Antoinette A. Grobbelaar, Naazneen Moolla, Patricia Leman, Jacqueline Weyer, Nadia Storm, Stewart D. McCulloch, Terence P. Scott, Wanda Markotter, Lieza Odendaal, Sarah J. Clift, Thomas W. Geisbert, Martin J. Hale, Alan Kemp

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Egyptian fruit bats (Rousettus aegyptiacus) were inoculated subcutaneously (n = 22) with Marburg virus (MARV). No deaths, overt signs of morbidity, or gross lesions was identified, but microscopic pathological changes were seen in the liver of infected bats. The virus was detected in 15 different tissues and plasma but only sporadically in mucosal swab samples, urine, and fecal samples. Neither seroconversion nor viremia could be demonstrated in any of the in-contact susceptible bats (n = 14) up to 42 days after exposure to infected bats. In bats rechallenged (n = 4) on day 48 after infection, there was no viremia, and the virus could not be isolated from any of the tissues tested. This study confirmed that infection profiles are consistent with MARV replication in a reservoir host but failed to demonstrate MARV transmission through direct physical contact or indirectly via air. Bats develop strong protective immunity after infection with MARV.

Original language	English (US)
Pages (from-to)	S109-S118
Journal	Journal of Infectious Diseases
Volume	212
DOIs	https://doi.org/10.1093/infdis/jiv132
State	Published - Oct 1 2015

Keywords

Egyptian fruit bat
Marburg virus
experimental infection
horizontal transmission
shedding

ASJC Scopus subject areas

General Medicine

Access to Document

10.1093/infdis/jiv132

Cite this

Paweska, J. T., Van Vuren, P. J., Fenton, K. A., Graves, K., Grobbelaar, A. A., Moolla, N., Leman, P., Weyer, J., Storm, N., McCulloch, S. D., Scott, T. P., Markotter, W., Odendaal, L., Clift, S. J., Geisbert, T. W., Hale, M. J., & Kemp, A. (2015). Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats. Journal of Infectious Diseases, 212, S109-S118. https://doi.org/10.1093/infdis/jiv132

Paweska, JT, Van Vuren, PJ, Fenton, KA, Graves, K, Grobbelaar, AA, Moolla, N, Leman, P, Weyer, J, Storm, N, McCulloch, SD, Scott, TP, Markotter, W, Odendaal, L, Clift, SJ, Geisbert, TW, Hale, MJ & Kemp, A 2015, 'Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats', Journal of Infectious Diseases, vol. 212, pp. S109-S118. https://doi.org/10.1093/infdis/jiv132

@article{dce6917174da4f06a2add9b4c45cb2d6,

title = "Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats",

abstract = "Egyptian fruit bats (Rousettus aegyptiacus) were inoculated subcutaneously (n = 22) with Marburg virus (MARV). No deaths, overt signs of morbidity, or gross lesions was identified, but microscopic pathological changes were seen in the liver of infected bats. The virus was detected in 15 different tissues and plasma but only sporadically in mucosal swab samples, urine, and fecal samples. Neither seroconversion nor viremia could be demonstrated in any of the in-contact susceptible bats (n = 14) up to 42 days after exposure to infected bats. In bats rechallenged (n = 4) on day 48 after infection, there was no viremia, and the virus could not be isolated from any of the tissues tested. This study confirmed that infection profiles are consistent with MARV replication in a reservoir host but failed to demonstrate MARV transmission through direct physical contact or indirectly via air. Bats develop strong protective immunity after infection with MARV.",

keywords = "Egyptian fruit bat, Marburg virus, experimental infection, horizontal transmission, shedding",

author = "Paweska, {Janusz T.} and {Van Vuren}, {Petrus Jansen} and Fenton, {Karla A.} and Kerry Graves and Grobbelaar, {Antoinette A.} and Naazneen Moolla and Patricia Leman and Jacqueline Weyer and Nadia Storm and McCulloch, {Stewart D.} and Scott, {Terence P.} and Wanda Markotter and Lieza Odendaal and Clift, {Sarah J.} and Geisbert, {Thomas W.} and Hale, {Martin J.} and Alan Kemp",

note = "Publisher Copyright: {\textcopyright} 2015 The Author 2015.",

year = "2015",

month = oct,

day = "1",

doi = "10.1093/infdis/jiv132",

language = "English (US)",

volume = "212",

pages = "S109--S118",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats

AU - Paweska, Janusz T.

AU - Van Vuren, Petrus Jansen

AU - Fenton, Karla A.

AU - Graves, Kerry

AU - Grobbelaar, Antoinette A.

AU - Moolla, Naazneen

AU - Leman, Patricia

AU - Weyer, Jacqueline

AU - Storm, Nadia

AU - McCulloch, Stewart D.

AU - Scott, Terence P.

AU - Markotter, Wanda

AU - Odendaal, Lieza

AU - Clift, Sarah J.

AU - Geisbert, Thomas W.

AU - Hale, Martin J.

AU - Kemp, Alan

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Egyptian fruit bats (Rousettus aegyptiacus) were inoculated subcutaneously (n = 22) with Marburg virus (MARV). No deaths, overt signs of morbidity, or gross lesions was identified, but microscopic pathological changes were seen in the liver of infected bats. The virus was detected in 15 different tissues and plasma but only sporadically in mucosal swab samples, urine, and fecal samples. Neither seroconversion nor viremia could be demonstrated in any of the in-contact susceptible bats (n = 14) up to 42 days after exposure to infected bats. In bats rechallenged (n = 4) on day 48 after infection, there was no viremia, and the virus could not be isolated from any of the tissues tested. This study confirmed that infection profiles are consistent with MARV replication in a reservoir host but failed to demonstrate MARV transmission through direct physical contact or indirectly via air. Bats develop strong protective immunity after infection with MARV.

AB - Egyptian fruit bats (Rousettus aegyptiacus) were inoculated subcutaneously (n = 22) with Marburg virus (MARV). No deaths, overt signs of morbidity, or gross lesions was identified, but microscopic pathological changes were seen in the liver of infected bats. The virus was detected in 15 different tissues and plasma but only sporadically in mucosal swab samples, urine, and fecal samples. Neither seroconversion nor viremia could be demonstrated in any of the in-contact susceptible bats (n = 14) up to 42 days after exposure to infected bats. In bats rechallenged (n = 4) on day 48 after infection, there was no viremia, and the virus could not be isolated from any of the tissues tested. This study confirmed that infection profiles are consistent with MARV replication in a reservoir host but failed to demonstrate MARV transmission through direct physical contact or indirectly via air. Bats develop strong protective immunity after infection with MARV.

KW - Egyptian fruit bat

KW - Marburg virus

KW - experimental infection

KW - horizontal transmission

KW - shedding

UR - http://www.scopus.com/inward/record.url?scp=84943253383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943253383&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiv132

DO - 10.1093/infdis/jiv132

M3 - Article

C2 - 25838270

AN - SCOPUS:84943253383

SN - 0022-1899

VL - 212

SP - S109-S118

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

ER -

Lack of Marburg Virus Transmission from Experimentally Infected to Susceptible In-Contact Egyptian Fruit Bats

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this