Lack of correlation between HERV-K expression and HIV-1 viral load in plasma specimens

Daniel Esqueda; Fangling Xu; Yuliana Moore; Zhen Yang; Gengming Huang; Patrick A. Lennon; Peter C. Hu; Jianli Dong

Lack of correlation between HERV-K expression and HIV-1 viral load in plasma specimens

Daniel Esqueda, Fangling Xu, Yuliana Moore, Zhen Yang, Gengming Huang, Patrick A. Lennon, Peter C. Hu, Jianli Dong

Pathology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of "contaminating" cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.

Original language	English (US)
Pages (from-to)	122-125
Number of pages	4
Journal	Annals of Clinical and Laboratory Science
Volume	43
Issue number	2
State	Published - 2013

Keywords

DNase I digestion
HERV-K expression
HIV-1 viral load

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Lack of correlation between HERV-K expression and HIV-1 viral load in plasma specimens",

abstract = "HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of {"}contaminating{"} cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.",

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TY - JOUR

T1 - Lack of correlation between HERV-K expression and HIV-1 viral load in plasma specimens

AU - Esqueda, Daniel

AU - Xu, Fangling

AU - Moore, Yuliana

AU - Yang, Zhen

AU - Huang, Gengming

AU - Lennon, Patrick A.

AU - Hu, Peter C.

AU - Dong, Jianli

PY - 2013

Y1 - 2013

N2 - HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of "contaminating" cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.

AB - HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of "contaminating" cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.

KW - DNase I digestion

KW - HERV-K expression

KW - HIV-1 viral load

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M3 - Article

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AN - SCOPUS:84878186586

SN - 0091-7370

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EP - 125

JO - Annals of Clinical and Laboratory Science

JF - Annals of Clinical and Laboratory Science

IS - 2

ER -

Lack of correlation between HERV-K expression and HIV-1 viral load in plasma specimens

Abstract

Keywords

ASJC Scopus subject areas

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