Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004

Clayton O. Onyango; Martin L. Opoka; Thomas G. Ksiazek; Pierre Formenty; Abdullahi Ahmed; Peter M. Tukei; Rosemary C. Sang; Victor O. Ofula; Samson L. Konongoi; Rodney L. Coldren; Thomas Grein; Dominique Legros; Mike Bell; Kevin M. De Cock; William J. Bellini; Jonathan S. Towner; Stuart T. Nichol; Pierre E. Rollin

doi:10.1086/520609

Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004

Clayton O. Onyango, Martin L. Opoka, Thomas G. Ksiazek, Pierre Formenty, Abdullahi Ahmed, Peter M. Tukei, Rosemary C. Sang, Victor O. Ofula, Samson L. Konongoi, Rodney L. Coldren, Thomas Grein, Dominique Legros, Mike Bell, Kevin M. De Cock, William J. Bellini, Jonathan S. Towner, Stuart T. Nichol, Pierre E. Rollin

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60 Scopus citations

Abstract

Between the months of April and June 2004, an Ebola hemorrhagic fever (EHF) outbreak was reported in Yambio county, southern Sudan. Blood samples were collected from a total of 36 patients with suspected EHF and were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G and M antibodies, antigen ELISA, and reverse-transcription polymerase chain reaction (PCR) of a segment of the Ebolavirus (EBOV) polymerase gene. A total of 13 patients were confirmed to be infected with EBOV. In addition, 4 fatal cases were classified as probable cases, because no samples were collected. Another 12 patients were confirmed to have acute measles infection during the same period that EBOV was circulating. Genetic analysis of PCR-positive samples indicated that the virus was similar to but distinct from Sudan EBOV Maleo 1979. In response, case management, social mobilization, and follow-up of contacts were set up as means of surveillance. The outbreak was declared to be over on 7 August 2004.

Original language	English (US)
Pages (from-to)	S193-S198
Journal	Journal of Infectious Diseases
Volume	196
Issue number	SUPPL. 2
DOIs	https://doi.org/10.1086/520609
State	Published - Nov 15 2007
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1086/520609

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Onyango, C. O., Opoka, M. L., Ksiazek, T. G., Formenty, P., Ahmed, A., Tukei, P. M., Sang, R. C., Ofula, V. O., Konongoi, S. L., Coldren, R. L., Grein, T., Legros, D., Bell, M., De Cock, K. M., Bellini, W. J., Towner, J. S., Nichol, S. T., & Rollin, P. E. (2007). Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004. Journal of Infectious Diseases, 196(SUPPL. 2), S193-S198. https://doi.org/10.1086/520609

Onyango, CO, Opoka, ML, Ksiazek, TG, Formenty, P, Ahmed, A, Tukei, PM, Sang, RC, Ofula, VO, Konongoi, SL, Coldren, RL, Grein, T, Legros, D, Bell, M, De Cock, KM, Bellini, WJ, Towner, JS, Nichol, ST & Rollin, PE 2007, 'Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004', Journal of Infectious Diseases, vol. 196, no. SUPPL. 2, pp. S193-S198. https://doi.org/10.1086/520609

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title = "Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004",

abstract = "Between the months of April and June 2004, an Ebola hemorrhagic fever (EHF) outbreak was reported in Yambio county, southern Sudan. Blood samples were collected from a total of 36 patients with suspected EHF and were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G and M antibodies, antigen ELISA, and reverse-transcription polymerase chain reaction (PCR) of a segment of the Ebolavirus (EBOV) polymerase gene. A total of 13 patients were confirmed to be infected with EBOV. In addition, 4 fatal cases were classified as probable cases, because no samples were collected. Another 12 patients were confirmed to have acute measles infection during the same period that EBOV was circulating. Genetic analysis of PCR-positive samples indicated that the virus was similar to but distinct from Sudan EBOV Maleo 1979. In response, case management, social mobilization, and follow-up of contacts were set up as means of surveillance. The outbreak was declared to be over on 7 August 2004.",

author = "Onyango, {Clayton O.} and Opoka, {Martin L.} and Ksiazek, {Thomas G.} and Pierre Formenty and Abdullahi Ahmed and Tukei, {Peter M.} and Sang, {Rosemary C.} and Ofula, {Victor O.} and Konongoi, {Samson L.} and Coldren, {Rodney L.} and Thomas Grein and Dominique Legros and Mike Bell and {De Cock}, {Kevin M.} and Bellini, {William J.} and Towner, {Jonathan S.} and Nichol, {Stuart T.} and Rollin, {Pierre E.}",

note = "Funding Information: Supplement sponsorship. This article was published as part of a supplement entitled “Filoviruses: Recent Advances and Future Challenges,” sponsored by the Public Health Agency of Canada, the National Institutes of Health, the Canadian Institutes of Health Research, Cangene, CUH2A, Smith Carter, Hemisphere Engineering, Crucell, and the International Centre for Infectious Diseases.",

year = "2007",

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doi = "10.1086/520609",

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T1 - Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004

AU - Onyango, Clayton O.

AU - Opoka, Martin L.

AU - Ksiazek, Thomas G.

AU - Formenty, Pierre

AU - Ahmed, Abdullahi

AU - Tukei, Peter M.

AU - Sang, Rosemary C.

AU - Ofula, Victor O.

AU - Konongoi, Samson L.

AU - Coldren, Rodney L.

AU - Grein, Thomas

AU - Legros, Dominique

AU - Bell, Mike

AU - De Cock, Kevin M.

AU - Bellini, William J.

AU - Towner, Jonathan S.

AU - Nichol, Stuart T.

AU - Rollin, Pierre E.

N1 - Funding Information: Supplement sponsorship. This article was published as part of a supplement entitled “Filoviruses: Recent Advances and Future Challenges,” sponsored by the Public Health Agency of Canada, the National Institutes of Health, the Canadian Institutes of Health Research, Cangene, CUH2A, Smith Carter, Hemisphere Engineering, Crucell, and the International Centre for Infectious Diseases.

PY - 2007/11/15

Y1 - 2007/11/15

N2 - Between the months of April and June 2004, an Ebola hemorrhagic fever (EHF) outbreak was reported in Yambio county, southern Sudan. Blood samples were collected from a total of 36 patients with suspected EHF and were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G and M antibodies, antigen ELISA, and reverse-transcription polymerase chain reaction (PCR) of a segment of the Ebolavirus (EBOV) polymerase gene. A total of 13 patients were confirmed to be infected with EBOV. In addition, 4 fatal cases were classified as probable cases, because no samples were collected. Another 12 patients were confirmed to have acute measles infection during the same period that EBOV was circulating. Genetic analysis of PCR-positive samples indicated that the virus was similar to but distinct from Sudan EBOV Maleo 1979. In response, case management, social mobilization, and follow-up of contacts were set up as means of surveillance. The outbreak was declared to be over on 7 August 2004.

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ER -

Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004

Abstract

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