Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

Jason Lanman; Tu Kiet T. Lam; Mark R. Emmett; Alan G. Marshall; Michael Sakalian; Peter E. Prevelige

doi:10.1038/nsmb790

Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

Jason Lanman, Tu Kiet T. Lam, Mark R. Emmett, Alan G. Marshall, Michael Sakalian, Peter E. Prevelige

Research output: Contribution to journal › Article › peer-review

138 Scopus citations

Abstract

To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

Original language	English (US)
Pages (from-to)	676-677
Number of pages	2
Journal	Nature Structural and Molecular Biology
Volume	11
Issue number	7
DOIs	https://doi.org/10.1038/nsmb790
State	Published - Jul 2004
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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title = "Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange",

abstract = "To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.",

author = "Jason Lanman and Lam, {Tu Kiet T.} and Emmett, {Mark R.} and Marshall, {Alan G.} and Michael Sakalian and Prevelige, {Peter E.}",

note = "Funding Information: This work was supported by grants from the US National Institutes of Health (AI44626) and the US National Science Foundation (CHE-99-09502), Florida State University and the US National High Magnetic Field Laboratory.",

year = "2004",

month = jul,

doi = "10.1038/nsmb790",

language = "English (US)",

volume = "11",

pages = "676--677",

journal = "Nature Structural and Molecular Biology",

issn = "1545-9993",

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T1 - Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

AU - Lanman, Jason

AU - Lam, Tu Kiet T.

AU - Emmett, Mark R.

AU - Marshall, Alan G.

AU - Sakalian, Michael

AU - Prevelige, Peter E.

N1 - Funding Information: This work was supported by grants from the US National Institutes of Health (AI44626) and the US National Science Foundation (CHE-99-09502), Florida State University and the US National High Magnetic Field Laboratory.

PY - 2004/7

Y1 - 2004/7

N2 - To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

AB - To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.

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JO - Nature Structural and Molecular Biology

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Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

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