TY - JOUR
T1 - Isolation of cationic peptides from rat polymorphonuclear leukocyte granule contents using fast protein liquid chromatography
AU - Loeffelholz, Michael J.
AU - Modrzakowski, Malcolm C.
N1 - Funding Information:
The authors thank the laboratory of Dr. Peter Johnson, Department of Chemistry, Ohio University, for assistance with, and the use of the Pharmacia FPLC system. This work was supported by Ohio University Baker Fund Award 84-12, and by research funds fim the Ohio University College of Osteopathic Medicine.
PY - 1986/11/1
Y1 - 1986/11/1
N2 - Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.
AB - Separation of extracted rat polymorphonuclear leukocyte (PMN) granule contents using fast protein liquid chromatography yielded four major protein fractions. These fractions consisted of myeloperoxidase (peak A), neutral protease (peak B), lysozyme (peak C), and low molecular weight, cationic peptides (peak D). This study represents the first noted purification of the cationic peptides of rat PMN granules.
KW - antimicrobial factors
KW - cationic proteins
KW - fast protein liquid chromatography
KW - leukocyte granules
KW - polymorphonuclear leukocyte
KW - rat
UR - http://www.scopus.com/inward/record.url?scp=0023034647&partnerID=8YFLogxK
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U2 - 10.1016/0003-2697(86)90564-6
DO - 10.1016/0003-2697(86)90564-6
M3 - Article
C2 - 3028207
AN - SCOPUS:0023034647
SN - 0003-2697
VL - 158
SP - 377
EP - 381
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -