Isolation and quantitative analysis of hydroxylysine glycosides

R. M. Napoli; B. S. Middleditch; N. M. Cintron; Y. M. Chen

doi:10.1007/BF02261068

Isolation and quantitative analysis of hydroxylysine glycosides

R. M. Napoli, B. S. Middleditch, N. M. Cintron, Y. M. Chen

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4 Scopus citations

Abstract

Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

Original language	English (US)
Pages (from-to)	497-501
Number of pages	5
Journal	Chromatographia
Volume	28
Issue number	9-10
DOIs	https://doi.org/10.1007/BF02261068
State	Published - Nov 1989
Externally published	Yes

Keywords

Amino acid derivatives
Collagen metabolites
Column liquid chromatography
Glycosides

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/BF02261068

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title = "Isolation and quantitative analysis of hydroxylysine glycosides",

abstract = "Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.",

keywords = "Amino acid derivatives, Collagen metabolites, Column liquid chromatography, Glycosides",

author = "Napoli, {R. M.} and Middleditch, {B. S.} and Cintron, {N. M.} and Chen, {Y. M.}",

year = "1989",

month = nov,

doi = "10.1007/BF02261068",

language = "English (US)",

volume = "28",

pages = "497--501",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer Vieweg",

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TY - JOUR

T1 - Isolation and quantitative analysis of hydroxylysine glycosides

AU - Napoli, R. M.

AU - Middleditch, B. S.

AU - Cintron, N. M.

AU - Chen, Y. M.

PY - 1989/11

Y1 - 1989/11

N2 - Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

AB - Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (using o-phthal-dialdehyde) and fluorescence detection.

KW - Amino acid derivatives

KW - Collagen metabolites

KW - Column liquid chromatography

KW - Glycosides

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DO - 10.1007/BF02261068

M3 - Article

AN - SCOPUS:0344853832

SN - 0009-5893

VL - 28

SP - 497

EP - 501

JO - Chromatographia

JF - Chromatographia

IS - 9-10

ER -

Isolation and quantitative analysis of hydroxylysine glycosides

Abstract

Keywords

ASJC Scopus subject areas

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