Abstract
Methods are described for the enzymatic isolation of endothelial cells from rat and rabbit mesenteric arteries and veins. The mesenteric vascular bed is incubated with an enzyme solution containing collagenase, deoxyribonuclease, papain, dithiothreitol and bovine serum albumin for 45 min at 37 °C in a shaking waterbath. After the 45 min digestion, cells are centrifuged and plated. This method yields an endothelial cell population with a high plating efficiency which is relatively free of smooth muscle contamination.
Original language | English (US) |
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Pages (from-to) | 257-262 |
Number of pages | 6 |
Journal | Methods in Cell Science |
Volume | 17 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1995 |
Externally published | Yes |
Keywords
- Culture
- Endothelial cells
- Isolation
- Mesentery
- Rabbit
- Rat
ASJC Scopus subject areas
- Cell Biology