Isolation and characterization of pirital virus, a newly discovered south American arenavirus

Charles F. Fulhorst; Michael D. Bowen; Rosa Alba Salas; Nuris M.C. De Manzione; Gloria Duno; Antonio Utrera; Thomas G. Ksiazek; C. J. Peters; Stuart T. Nichol; Edith De Miller; Duilia Tovar; Bricio Ramos; Clovis Vasquez; Robert B. Tesh

doi:10.4269/ajtmh.1997.56.548

Isolation and characterization of pirital virus, a newly discovered south American arenavirus

Charles F. Fulhorst, Michael D. Bowen, Rosa Alba Salas, Nuris M.C. De Manzione, Gloria Duno, Antonio Utrera, Thomas G. Ksiazek, C. J. Peters, Stuart T. Nichol, Edith De Miller, Duilia Tovar, Bricio Ramos, Clovis Vasquez, Robert B. Tesh

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Specific rodent species are principal hosts for each of the well- characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.

Original language	English (US)
Pages (from-to)	548-553
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	56
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.1997.56.548
State	Published - May 1997
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases
Virology
Parasitology

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10.4269/ajtmh.1997.56.548

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Fulhorst, C. F., Bowen, M. D., Salas, R. A., De Manzione, N. M. C., Duno, G., Utrera, A., Ksiazek, T. G., Peters, C. J., Nichol, S. T., De Miller, E., Tovar, D., Ramos, B., Vasquez, C., & Tesh, R. B. (1997). Isolation and characterization of pirital virus, a newly discovered south American arenavirus. American Journal of Tropical Medicine and Hygiene, 56(5), 548-553. https://doi.org/10.4269/ajtmh.1997.56.548

Fulhorst, CF, Bowen, MD, Salas, RA, De Manzione, NMC, Duno, G, Utrera, A, Ksiazek, TG, Peters, CJ, Nichol, ST, De Miller, E, Tovar, D, Ramos, B, Vasquez, C & Tesh, RB 1997, 'Isolation and characterization of pirital virus, a newly discovered south American arenavirus', American Journal of Tropical Medicine and Hygiene, vol. 56, no. 5, pp. 548-553. https://doi.org/10.4269/ajtmh.1997.56.548

@article{b697d72e33e840428a061601f51ce125,

title = "Isolation and characterization of pirital virus, a newly discovered south American arenavirus",

abstract = "Specific rodent species are principal hosts for each of the well- characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.",

author = "Fulhorst, {Charles F.} and Bowen, {Michael D.} and Salas, {Rosa Alba} and {De Manzione}, {Nuris M.C.} and Gloria Duno and Antonio Utrera and Ksiazek, {Thomas G.} and Peters, {C. J.} and Nichol, {Stuart T.} and {De Miller}, Edith and Duilia Tovar and Bricio Ramos and Clovis Vasquez and Tesh, {Robert B.}",

note = "Funding Information: Clinical methods. Neonates of >1000 g with severe hypoxemia (Pa02 <50 torr while breathing 100% oxygen), without structural congenital heart disease, and suspected clinically of having PPHN, were examined with one or more of the following confirmatory diagnostic procedures: Supported in part by the Children's Research Fund of Chicago Grant 693530, Block Fund Grant 6 3340 126094, and Clinical Research Center Grant 193. Submitted for publication Dec. 3, 1985; accepted Oct. 21, 1986. Reprint requests: Cathy Hammerman, M.D., Department of Pediatrics, Box 325, University of Chicago, 5825 Maryland Ave, Chicago, IL 60637.",

year = "1997",

month = may,

doi = "10.4269/ajtmh.1997.56.548",

language = "English (US)",

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pages = "548--553",

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T1 - Isolation and characterization of pirital virus, a newly discovered south American arenavirus

AU - Fulhorst, Charles F.

AU - Bowen, Michael D.

AU - Salas, Rosa Alba

AU - De Manzione, Nuris M.C.

AU - Duno, Gloria

AU - Utrera, Antonio

AU - Ksiazek, Thomas G.

AU - Peters, C. J.

AU - Nichol, Stuart T.

AU - De Miller, Edith

AU - Tovar, Duilia

AU - Ramos, Bricio

AU - Vasquez, Clovis

AU - Tesh, Robert B.

N1 - Funding Information: Clinical methods. Neonates of >1000 g with severe hypoxemia (Pa02 <50 torr while breathing 100% oxygen), without structural congenital heart disease, and suspected clinically of having PPHN, were examined with one or more of the following confirmatory diagnostic procedures: Supported in part by the Children's Research Fund of Chicago Grant 693530, Block Fund Grant 6 3340 126094, and Clinical Research Center Grant 193. Submitted for publication Dec. 3, 1985; accepted Oct. 21, 1986. Reprint requests: Cathy Hammerman, M.D., Department of Pediatrics, Box 325, University of Chicago, 5825 Maryland Ave, Chicago, IL 60637.

PY - 1997/5

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N2 - Specific rodent species are principal hosts for each of the well- characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.

AB - Specific rodent species are principal hosts for each of the well- characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.

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Isolation and characterization of pirital virus, a newly discovered south American arenavirus

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