TY - JOUR
T1 - Isolation and characterization of ovine gamma globulins
AU - Aalund, Ole
AU - Osebold, John W.
AU - Murphy, Frederick A.
N1 - Funding Information:
1 These studies were supported in part, b> research grant A-1080 from the National Institutes of Health, IT. S. Public Health Service. 2 Present address : Hygienic-Ractrriological Laboratory of the Royal 1’cterinary and Agricul- tural College, Bidowsvej 13, Copenhagen T-. Denmark.
PY - 1965/1
Y1 - 1965/1
N2 - Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.
AB - Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.
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U2 - 10.1016/0003-9861(65)90299-7
DO - 10.1016/0003-9861(65)90299-7
M3 - Article
C2 - 14281939
AN - SCOPUS:50549199866
SN - 0003-9861
VL - 109
SP - 142
EP - 149
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -