Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C

Eugene Kulikov, Andrew M. Kropinski, Alla Golomidova, Erika Lingohr, Vadim Govorun, Marina Serebryakova, Nikolai Prokhorov, Maria Letarova, Anatolij Manykin, Alexandra Strotskaya, Andrey Letarov

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Lytic coliphage vB_EcoP_G7C and several other highly related isolates were obtained repeatedly from the samples of horse feces held in the same stable thus representing a component of the normal indigenous intestinal communities in this population of animals. The genome of G7C consists of 71,759. bp with terminal repeats of about 1160. bp, yielding approximately 73 kbp packed DNA size. Seventy-eight potential open reading frames, most of them unique to N4-like viruses, were identified and annotated. The overall layout of functional gene groups was close to that of the original N4 phage, with some important changes in late gene area including new tail fiber proteins containing hydrolytic domains. Structural proteome analysis confirmed all the predicted subunits of the viral particle. Unlike N4 itself, phage G7C did not exhibit a lysis-inhibited phenotype.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
Issue number2
StatePublished - May 10 2012


  • Complete genome sequence
  • Enterobacteria bacteriophage
  • Escherichia coli
  • Lytic phage
  • N4
  • N4-like coliphage
  • Podoviridae
  • Proteomics
  • Sequence analysis
  • Tail fiber

ASJC Scopus subject areas

  • Virology


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