TY - JOUR
T1 - Intragraft Gene Expression Profile During Acute Cellular Rejection in Clinical Small Bowel Transplantation
T2 - A Case Report
AU - Bradley, S. P.
AU - Pahari, M.
AU - Elias, G.
AU - Uknis, M. E.
AU - Misra, M. V.
AU - Rastellini, C.
AU - Cicalese, L.
PY - 2006/7
Y1 - 2006/7
N2 - Background: Acute cellular rejection (ACR) is a common complication of small bowel transplantation (SBTx) and the major cause of graft loss. However, little is known regarding the genetic graft response to ACR in clinical transplants. In this study, we have determined a genetic expression profile of intestinal graft response to ACR after living related (LR) SBTx. Results: By identifying the expression profiles of reported markers of rejection we were able to identify 57 genes that had significantly increased (more than twofold) expression in response to ACR. Known markers of rejection identified: MMP-9, MMP-2, VIP, IFNγ, IL-2R, MADCAM-1, HSP-60, and HSP-70 all had greater than twofold increased expression after ACR diagnosed (week 3 to week 6). The newly identified genes were: IFI27, EPST11, APAF1, LAP3, STK6, and MDK. Conclusion: Newly identified up-regulated genes in response to ACR in small bowel graft are involved in the immune response, cell adhesion, neurogenesis, cell division and proliferation, DNA replication/repair, protein ubiqutin/proteolysis, and apoptosis. TNFα up-regulated early at week 2 biopsy may be an early genetic marker of ACR in SBTx.
AB - Background: Acute cellular rejection (ACR) is a common complication of small bowel transplantation (SBTx) and the major cause of graft loss. However, little is known regarding the genetic graft response to ACR in clinical transplants. In this study, we have determined a genetic expression profile of intestinal graft response to ACR after living related (LR) SBTx. Results: By identifying the expression profiles of reported markers of rejection we were able to identify 57 genes that had significantly increased (more than twofold) expression in response to ACR. Known markers of rejection identified: MMP-9, MMP-2, VIP, IFNγ, IL-2R, MADCAM-1, HSP-60, and HSP-70 all had greater than twofold increased expression after ACR diagnosed (week 3 to week 6). The newly identified genes were: IFI27, EPST11, APAF1, LAP3, STK6, and MDK. Conclusion: Newly identified up-regulated genes in response to ACR in small bowel graft are involved in the immune response, cell adhesion, neurogenesis, cell division and proliferation, DNA replication/repair, protein ubiqutin/proteolysis, and apoptosis. TNFα up-regulated early at week 2 biopsy may be an early genetic marker of ACR in SBTx.
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U2 - 10.1016/j.transproceed.2006.05.054
DO - 10.1016/j.transproceed.2006.05.054
M3 - Article
C2 - 16908268
AN - SCOPUS:33747063318
SN - 0041-1345
VL - 38
SP - 1740
EP - 1741
JO - Transplantation proceedings
JF - Transplantation proceedings
IS - 6
ER -