TY - JOUR
T1 - Interference mapping of nuclear protein binding to the acute phase response element of the mouse α1 acid glycoprotein gene
AU - Rabek, Jeffrey P.
AU - Papaconstantinou, John
N1 - Funding Information:
Publication No. 101 supported by U.S.P.H.S. Grant PO2 AG10514 awarded by the National Institute on Aging and Grant 8540 supported by the Shriners Burns Institute.
PY - 1999/2/24
Y1 - 1999/2/24
N2 - Exchange in binding of transcription factors C/EBPα and C/EBPβ at a regulatory site in the α1-acid glycoprotein (AGP) promoter, termed the acute phase response element (APRE), has been correlated with bacterial lipopolysaccharide (LPS) mediated induction. The APRE contains overlapping recognition sequences for C/EBP's and glucocorticoid receptor (GR). Electrophortetic mobility shift assays show that this site can bind both GR and C/EBP. However, using liver nuclear extract, which contains GR binding activity, only C/EBP binds to the APRE. Binding interference methods, using dimethyl sulfate and potassium permanganate modification of specific bases, detected interference only with modification of bases that are in the region of the C/EBP binding site that do not overlap with the GRE sequence. There are no significant differences between the interference patterns of control and LPS treated liver nuclear extracts, suggesting that the region of close contact between protein and DNA is similar for C/EBPα (untreated) and C/EBPβ (treated).
AB - Exchange in binding of transcription factors C/EBPα and C/EBPβ at a regulatory site in the α1-acid glycoprotein (AGP) promoter, termed the acute phase response element (APRE), has been correlated with bacterial lipopolysaccharide (LPS) mediated induction. The APRE contains overlapping recognition sequences for C/EBP's and glucocorticoid receptor (GR). Electrophortetic mobility shift assays show that this site can bind both GR and C/EBP. However, using liver nuclear extract, which contains GR binding activity, only C/EBP binds to the APRE. Binding interference methods, using dimethyl sulfate and potassium permanganate modification of specific bases, detected interference only with modification of bases that are in the region of the C/EBP binding site that do not overlap with the GRE sequence. There are no significant differences between the interference patterns of control and LPS treated liver nuclear extracts, suggesting that the region of close contact between protein and DNA is similar for C/EBPα (untreated) and C/EBPβ (treated).
UR - http://www.scopus.com/inward/record.url?scp=0033599351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033599351&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0240
DO - 10.1006/bbrc.1999.0240
M3 - Article
C2 - 10049758
AN - SCOPUS:0033599351
SN - 0006-291X
VL - 255
SP - 608
EP - 613
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -