Abstract
The aim of the present study was to evaluate in vivo the role of polyamines in the secretion of atrial natriuretic peptide (ANP). α-Difluoromethylornithine (DFMO) which inhibits ornithine decarboxylase activity and polyamine synthesis was given in drinking water and through intraperitoneal administration to Sprague-Dawley rats. Carotid artery was cannulated for collection of blood samples and measurement of blood pressure following the administration of arginine-vasopressin (AVP). Analysis of polyamines in cardiac tissue indicated that DFMO treatment decreased contents of putrescine and spermidine in cardiac tissue by 80% and 48%, respectively. Quantitation of ANP in plasma by radioimmunoassay indicated that both basal and stimulated levels of ANP in DFMO-treated animals were 21.5% and 50% of those in control rats. The administration of putrescine restored the levels of basal and AVP-stimulated levels of ANP in plasma which confirmed that DFMO effect on ANP secretion occurred specifically through the polyamine pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 227-236 |
| Number of pages | 10 |
| Journal | Regulatory Peptides |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 18 1992 |
| Externally published | Yes |
Keywords
- Atrial natriuretic peptide
- DFMO
- Polyamine
ASJC Scopus subject areas
- Endocrinology
- Cellular and Molecular Neuroscience
- Physiology
- Biochemistry
- Clinical Biochemistry