Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III

Jingjing Fan; Yi Liu; Xuping Xie; Bo Zhang; Zhiming Yuan

doi:10.1007/s12250-013-3331-8

Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III

Jingjing Fan, Yi Liu, Xuping Xie, Bo Zhang, Zhiming Yuan

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models.

Original language	English (US)
Pages (from-to)	152-160
Number of pages	9
Journal	Virologica Sinica
Volume	28
Issue number	3
DOIs	https://doi.org/10.1007/s12250-013-3331-8
State	Published - Jun 2013
Externally published	Yes

Keywords

Antibody
Cross-protection
E protein domain III
Japanese encephalitis virus

ASJC Scopus subject areas

Infectious Diseases
Molecular Medicine
Virology
Immunology

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title = "Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III",

abstract = "Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models.",

keywords = "Antibody, Cross-protection, E protein domain III, Japanese encephalitis virus",

author = "Jingjing Fan and Yi Liu and Xuping Xie and Bo Zhang and Zhiming Yuan",

note = "Funding Information: We thank Dr. Philippe Despres for providing DENV1, DENV3, and DENV4 plasmids, Dr. Rory Marks for providing the DENV2 plasmid and Dr. Pei-Yong Shi for providing the WNV plasmid as the templates used in our study. This work was supported by Important National Science & Technology Specific Projects (2012ZX10004403, 2012ZX10004219), National Natural Scientific Fund of China (81072675).",

year = "2013",

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doi = "10.1007/s12250-013-3331-8",

language = "English (US)",

volume = "28",

pages = "152--160",

journal = "Virologica Sinica",

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TY - JOUR

T1 - Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III

AU - Fan, Jingjing

AU - Liu, Yi

AU - Xie, Xuping

AU - Zhang, Bo

AU - Yuan, Zhiming

N1 - Funding Information: We thank Dr. Philippe Despres for providing DENV1, DENV3, and DENV4 plasmids, Dr. Rory Marks for providing the DENV2 plasmid and Dr. Pei-Yong Shi for providing the WNV plasmid as the templates used in our study. This work was supported by Important National Science & Technology Specific Projects (2012ZX10004403, 2012ZX10004219), National Natural Scientific Fund of China (81072675).

PY - 2013/6

Y1 - 2013/6

N2 - Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models.

AB - Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models.

KW - Antibody

KW - Cross-protection

KW - E protein domain III

KW - Japanese encephalitis virus

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Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III

Abstract

Keywords

ASJC Scopus subject areas

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