Influence of dark phase restricted high fat feeding on myocardial adaptation in mice

Ju Yun Tsai, Carolina Villegas-Montoya, Brandon B. Boland, Zachary Blasier, Oluwaseun Egbejimi, Raquel Gonzalez, Michael Kueht, Tracy A. McElfresh, Rachel A. Brewer, Margaret P. Chandler, Molly S. Bray, Martin E. Young

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Prolonged high fat feeding is associated with myocardial contractile dysfunction in rodents. However, epidemiological data do not necessarily support the concept that fat-enriched diets adversely affect cardiac function in humans. When fed in an ad libitum manner, laboratory rodents consume chow throughout the day. In contrast, humans typically consume food only during the awake phase. Discrepancies between rodent and human feeding behaviors led us to hypothesize that the time of day at which dietary lipids are consumed significantly influences myocardial adaptation. In order to better mimic feeding behavior in humans, mice were fed (either a control or high fat diet) only during the 12-hour dark phase (i.e., no food was provided during the light phase). We report that compared to dark phase restricted control diet fed mice, mice fed a high fat diet during the dark phase exhibit: 1) essentially normal body weight gain and energy balance; 2) increased fatty acid oxidation at whole body, as well as skeletal and cardiac muscle (in the presence of insulin and/or at high workloads) levels; 3) induction of fatty acid responsive genes, including genes promoting triglyceride turnover in the heart; 4) no evidence of cardiac hypertrophy; and 5) persistence/improvement of myocardial contractile function, as assessed ex vivo. These data are consistent with the hypothesis that ingestion of dietary fat only during the more active/awake period allows adequate metabolic adaptation, thereby preserving myocardial contractile function. This article is part of a Special Issue entitled "Focus on cardiac metabolism".

Original languageEnglish (US)
Pages (from-to)147-155
Number of pages9
JournalJournal of Molecular and Cellular Cardiology
Issue number1
StatePublished - Feb 2013
Externally publishedYes


  • Gene expression
  • High fat feeding
  • Metabolism
  • Triglyceride

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Influence of dark phase restricted high fat feeding on myocardial adaptation in mice'. Together they form a unique fingerprint.

Cite this