Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

Kota V. Ramana; Krishan K. Kohli

doi:10.3109/01480549609002199

Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

Kota V. Ramana, Krishan K. Kohli

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

An intraperitoneal administration of PB at a daily dose of 50 mg Kg^-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b₅ and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.

Original language	English (US)
Pages (from-to)	97-107
Number of pages	11
Journal	Drug and Chemical Toxicology
Volume	19
Issue number	1-2
DOIs	https://doi.org/10.3109/01480549609002199
State	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Toxicology
Pharmacology
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis
Chemical Health and Safety

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10.3109/01480549609002199

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title = "Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)",

abstract = "An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.",

author = "Ramana, {Kota V.} and Kohli, {Krishan K.}",

note = "Funding Information: We wish to acknowledge Rhone-Poulenc (India) Limited, Bombay, India for the supply of PB. This work was supported by a research grant 4-14-92-G from the Department of Atomic Energy, Bombay, India.",

year = "1996",

doi = "10.3109/01480549609002199",

language = "English (US)",

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pages = "97--107",

journal = "Drug and Chemical Toxicology",

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T1 - Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

AU - Ramana, Kota V.

AU - Kohli, Krishan K.

N1 - Funding Information: We wish to acknowledge Rhone-Poulenc (India) Limited, Bombay, India for the supply of PB. This work was supported by a research grant 4-14-92-G from the Department of Atomic Energy, Bombay, India.

PY - 1996

Y1 - 1996

N2 - An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.

AB - An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.

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Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

Abstract

ASJC Scopus subject areas

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