TY - JOUR
T1 - Induction of chromosome aberrations in lymphocytes of mice after subchronic exposure to benzene
AU - Rithidech, Kanokporn
AU - Au, William W.
AU - Sadagopa Ramanujam, V. M.
AU - Whorton, Elbert B.
AU - Legator, Marvin S.
N1 - Funding Information:
The investigation was partly supported by the Texas Air Control Board (TACB), Environmetal Protection Agency through Contract number . R810920-01, and an Intramural Grant from the University of Texas Medical Branch, Galveston, TX.
PY - 1987/6
Y1 - 1987/6
N2 - The induction of chromosome aberrations in lymphocytes of mice after subchronic exposure to benzene was investigated. 4 groups of 5 Swiss (ICR) male mice were given orally a solution of benzene every day for 14 days except days 5 and 10. The daily doses were 0, 36.6, 73.2 and 146.4 mg/kg. Mice were sacrificed on day 15, lymphocytes were obtained by perfusion of the spleen and the cells were cultured in RPMI 1640 medium. After 48 h of culture, cells were harvested for cytogenetic analysis. A significant dose-dependent increase in the frequency of cells with chromatid aberrations were found (p < 0.001). A significant increase in polyploid cells were also observed (p ≤ 0.05). This study represents the first report on the induction of chromosome aberrations and polyploid cells in lymphocytes of mice after subchronic exposure to benzene. Such dual activity of benzene suggests that benzene may be responsible for more human health problems than currently estimated.
AB - The induction of chromosome aberrations in lymphocytes of mice after subchronic exposure to benzene was investigated. 4 groups of 5 Swiss (ICR) male mice were given orally a solution of benzene every day for 14 days except days 5 and 10. The daily doses were 0, 36.6, 73.2 and 146.4 mg/kg. Mice were sacrificed on day 15, lymphocytes were obtained by perfusion of the spleen and the cells were cultured in RPMI 1640 medium. After 48 h of culture, cells were harvested for cytogenetic analysis. A significant dose-dependent increase in the frequency of cells with chromatid aberrations were found (p < 0.001). A significant increase in polyploid cells were also observed (p ≤ 0.05). This study represents the first report on the induction of chromosome aberrations and polyploid cells in lymphocytes of mice after subchronic exposure to benzene. Such dual activity of benzene suggests that benzene may be responsible for more human health problems than currently estimated.
KW - Benzene, subchronic exposure
KW - Chromosome aberrations: (Mouse)
KW - Lymphocytes
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U2 - 10.1016/0165-1218(87)90102-9
DO - 10.1016/0165-1218(87)90102-9
M3 - Article
C2 - 3587261
AN - SCOPUS:0023195910
SN - 0165-1218
VL - 188
SP - 135
EP - 140
JO - Mutation Research/Genetic Toxicology
JF - Mutation Research/Genetic Toxicology
IS - 2
ER -