TY - JOUR
T1 - Induction of a subgroup of acute phase protein genes in mouse liver by hyperthermia
AU - Yiangou, Minas
AU - Paraskeva, Efrosini
AU - Hsieh, Ching Chyuan
AU - Markou, Eustathia
AU - Victoratos, Panayiotis
AU - Scouras, Zacharias
AU - Papaconstantinou, John
N1 - Funding Information:
This investigation was supported by a grant from the Shriners Burns Institute, Galveston Unit (awarded to J.P.) and a grant from the Hellenic General Secretary of Research and Technology (awarded to M.Y.) We acknowledge the assistance of M.A. Ridgeway in the preparation of the manuscript and of Dr. David Konkel for his review of the manuscript.
PY - 1998/3/9
Y1 - 1998/3/9
N2 - We have demonstrated that two members of the acute phase reactant family of positively regulated genes, α1-acid glycoprotein (AGP-1 and AGP-2) and C-reactive protein (CRP) are induced by hyperthermia, while two others, the serum amyloid A (SAA) and α1-antitrypsin (AT) genes, are not. Albumin (ALB), a negative acute phase reactant gene, is also induced by hyperthermia. The AGP-1, AGP-2, and CRP genes require glucocorticoids, but not IL-6, IL- 1β or TNFα in response to hyperthermia. As with LPS, the C/EBPβ mRNA levels increased, while the C/EBPα mRNA levels decreased in response to LPS. In contrast to the LPS response, C/EBPδ was unchanged. Protein pool levels and DNA-binding activities of the 35 and 20 kDa C/EBPβ isoforms increase, whereas protein pool levels of the 42 kDa C/EBPα decrease and the 30 kDa remained high. These studies suggest that the synthesis of specific C/EBPα and C/EBPβ isoforms is induced by hyperthermia, and that the regulation of the AGP-1 and AGP-2 genes during heat stress may involve one of these isoforms. The difference between the responses to hyperthermia and LPS is that the former, may not involve the participation of cytokines. Furthermore, since cis-acting heat shock elements (HSE) are located in the promoter regions of the ALB, CRP, and C/EBPβ genes, these regulatory sequences may be involved in the in vivo activation of these genes by hyperthermia.
AB - We have demonstrated that two members of the acute phase reactant family of positively regulated genes, α1-acid glycoprotein (AGP-1 and AGP-2) and C-reactive protein (CRP) are induced by hyperthermia, while two others, the serum amyloid A (SAA) and α1-antitrypsin (AT) genes, are not. Albumin (ALB), a negative acute phase reactant gene, is also induced by hyperthermia. The AGP-1, AGP-2, and CRP genes require glucocorticoids, but not IL-6, IL- 1β or TNFα in response to hyperthermia. As with LPS, the C/EBPβ mRNA levels increased, while the C/EBPα mRNA levels decreased in response to LPS. In contrast to the LPS response, C/EBPδ was unchanged. Protein pool levels and DNA-binding activities of the 35 and 20 kDa C/EBPβ isoforms increase, whereas protein pool levels of the 42 kDa C/EBPα decrease and the 30 kDa remained high. These studies suggest that the synthesis of specific C/EBPα and C/EBPβ isoforms is induced by hyperthermia, and that the regulation of the AGP-1 and AGP-2 genes during heat stress may involve one of these isoforms. The difference between the responses to hyperthermia and LPS is that the former, may not involve the participation of cytokines. Furthermore, since cis-acting heat shock elements (HSE) are located in the promoter regions of the ALB, CRP, and C/EBPβ genes, these regulatory sequences may be involved in the in vivo activation of these genes by hyperthermia.
KW - Acute phase response
KW - C-reactive protein
KW - C/EBP
KW - Heat shock
KW - α-Acid glycoprotein
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U2 - 10.1016/S0167-4781(97)00188-7
DO - 10.1016/S0167-4781(97)00188-7
M3 - Article
C2 - 9540835
AN - SCOPUS:0032498833
SN - 0167-4781
VL - 1396
SP - 191
EP - 206
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2
ER -