Increased organ blood flow in chronic endotoxemia is reversed by nitric oxide synthase inhibition

J. Meyer, F. Hinder, J. Stothert, L. D. Traber, D. N. Herndon, J. T. Flynn, D. L. Traber

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


We evaluated regional blood flows in a hyperdynamic sepsis model and the reversal of increased flows by blockade of nitric oxide (NO) synthase. Seven awake sheep were continuously infused with Escherichia coli endotoxin [lipopolysaccharide (LPS), 10 ng · kg-1 · min-1] for 48 h. The NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME, 25 mg/kg) was injected after 24 h. Blood flows to systemic organs were determined with the radioactive microsphere technique. LPS induced elevation of cardiac index by 36% (P < 0.05) and a fall in systemic vascular resistance index by 37% (P < 0.05) at 0 h [time of L-NAME administration, 24 h after infusion of LPS had begun]. L-NAME administration normalized cardiac index [6.1 ± 0.5 at 4 h posttreatment, 6.1 ± 0.51 · min-1 · m-2 at -24 h (baseline)] and systemic vascular resistance index (1,333 ± 105 at 4 h posttreatment, 1,280 ± 163 dyn · s · cm-5 · m2 at -24 h) and reduced all regional blood flows to near-baseline levels for the remainder of the study period (24 h). O2 consumption was unaffected by treatment.

Original languageEnglish (US)
Pages (from-to)2785-2793
Number of pages9
JournalJournal of Applied Physiology
Issue number6
StatePublished - 1994
Externally publishedYes


  • acute lung injury
  • cardiopulmonary
  • endotoxin
  • lung
  • nitric oxide
  • oxygen consumption
  • pulmonary
  • pulmonary hemodynamics
  • radioactive microsphere technique
  • regional blood flow
  • sepsis

ASJC Scopus subject areas

  • General Medicine


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