TY - GEN
T1 - In vivo effects of an inhibitor of nuclear factor-kappa B on thrombogenic properties of antiphospholipid antibodies
AU - Montiel-Manzano, G.
AU - Romay-Penabad, Z.
AU - De Martínez, E. Papalardo
AU - Meillon-García, L. A.
AU - García-Latorre, E.
AU - Reyes-Maldonado, E.
AU - Pierangeli, Silvia S.
PY - 2007/6
Y1 - 2007/6
N2 - It has been shown that endothelial cell (EC) activation and tissue factor (TF) upregulation in EC and monocytes by antiphospholipid antibodies (aPL Abs) leads to a prothrombotic state and involves translocation of nuclear factor-kappa B (NF-κB). Here we examined the effects of an NF-κB inhibitor on aPL-induced thrombosis, TF activity, and EC in vivo. We treated CD1 mice with IgG from a patient with antiphospholipid syndrome (IgG-APS) or with control IgG (IgG-NHS). The adhesion of leukocytes (number of white blood cells) to EC in cremaster muscle (as an indication of EC activation) as well as the size of an induced thrombus in the femoral vein of the mice were examined. Some mice in each group were infused with 10 μM MG132 (an inhibitor of NF-κB). TF activity was determined using a chromogenic assay in homogenates of carotid arteries and in peritoneal cells of mice. In vivo, IgG-APS increased significantly the number of white blood cells adhering to ECs (4.7 ± 2.2) when compared to control mice (1.5 ± 0.8), and these effects were significantly reduced when mice were pretreated with MG132 (0.8 ± 0.2). IgG-APS increased significantly the thrombus size and MG132 inhibited that effect (93%). Treatment of the mice with IgG-APS also induced significantly increased TF function in peritoneal cells and in homogenates of carotid arteries. Pretreatment of the mice with MG132 abrogated those effects significantly. Mice injected with IgG-APS or with IgM-APS with or without the inhibitor had medium-high titers of anticardiolipin antibodies in serum at the time of the surgical procedures. The data show that prothrombotic and proinflammatory properties of IgG-APS and IgM-APS are downregulated in vivo by an NF-κB inhibitor. These findings may be important in designing new modalities of targeted therapies to treat thrombosis in patients with APS.
AB - It has been shown that endothelial cell (EC) activation and tissue factor (TF) upregulation in EC and monocytes by antiphospholipid antibodies (aPL Abs) leads to a prothrombotic state and involves translocation of nuclear factor-kappa B (NF-κB). Here we examined the effects of an NF-κB inhibitor on aPL-induced thrombosis, TF activity, and EC in vivo. We treated CD1 mice with IgG from a patient with antiphospholipid syndrome (IgG-APS) or with control IgG (IgG-NHS). The adhesion of leukocytes (number of white blood cells) to EC in cremaster muscle (as an indication of EC activation) as well as the size of an induced thrombus in the femoral vein of the mice were examined. Some mice in each group were infused with 10 μM MG132 (an inhibitor of NF-κB). TF activity was determined using a chromogenic assay in homogenates of carotid arteries and in peritoneal cells of mice. In vivo, IgG-APS increased significantly the number of white blood cells adhering to ECs (4.7 ± 2.2) when compared to control mice (1.5 ± 0.8), and these effects were significantly reduced when mice were pretreated with MG132 (0.8 ± 0.2). IgG-APS increased significantly the thrombus size and MG132 inhibited that effect (93%). Treatment of the mice with IgG-APS also induced significantly increased TF function in peritoneal cells and in homogenates of carotid arteries. Pretreatment of the mice with MG132 abrogated those effects significantly. Mice injected with IgG-APS or with IgM-APS with or without the inhibitor had medium-high titers of anticardiolipin antibodies in serum at the time of the surgical procedures. The data show that prothrombotic and proinflammatory properties of IgG-APS and IgM-APS are downregulated in vivo by an NF-κB inhibitor. These findings may be important in designing new modalities of targeted therapies to treat thrombosis in patients with APS.
KW - Antiphospholipid antibodies
KW - Antiphospholipid syndrome
KW - Endothelial cells
KW - NF-κB
KW - Thrombosis
UR - http://www.scopus.com/inward/record.url?scp=34948875756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34948875756&partnerID=8YFLogxK
U2 - 10.1196/annals.1422.057
DO - 10.1196/annals.1422.057
M3 - Conference contribution
C2 - 17894019
AN - SCOPUS:34948875756
SN - 157331708X
SN - 9781573317085
T3 - Annals of the New York Academy of Sciences
SP - 540
EP - 553
BT - Autoimmunity, Part D
PB - Blackwell Publishing Inc.
ER -