In vitro transcription/translation system: A versatile tool in the search for missing proteins

Péter Horvatovich, Ákos Végvári, Justin Saul, Jin G. Park, Ji Qiu, Michael Syring, Patrick Pirrotte, Konstantinos Petritis, Tony J. Tegeler, Meraj Aziz, Manuel Fuentes, Paula Diez, Maria Gonzalez-Gonzalez, Nieves Ibarrola, Conrad Droste, Javier De Las Rivas, Concha Gil, Felipe Clemente, Maria Luisa Hernaez, Fernando J. CorralesCarol L. Nilsson, Frode S. Berven, Rainer Bischoff, Thomas E. Fehniger, Joshua LaBaer, György Marko-Varga

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations


Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria established by neXtProt, and are considered to be "missing" proteins. One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to identify as many of these missing proteins as possible in human samples using mass spectrometry-based methods. To further this goal, a consortium of C-HPP teams (chromosomes 5, 10, 16, and 19) has joined forces to devise new strategies to identify missing proteins by use of a cell-free in vitro transcription/translation system (IVTT). The proposed strategy employs LC-MS/MS data-dependent acquisition (DDA) and targeted selective reaction monitoring (SRM) methods to scrutinize low-complexity samples derived from IVTT. The optimized assays are then applied to identify missing proteins in human cells and tissues. We describe the approach and show proof-of-concept results for development of LC-SRM assays for identification of 18 missing proteins. We believe that the IVTT system, when coupled with downstream mass spectrometric identification, can be applied to identify proteins that have eluded more traditional methods of detection.

Original languageEnglish (US)
Pages (from-to)3441-3451
Number of pages11
JournalJournal of Proteome Research
Issue number9
StatePublished - Sep 4 2015


  • Chromosome-Centric Human Proteome Project
  • LC-MS
  • Missing proteins
  • bioinformatics
  • in vitro transcription/translation system
  • proteomics

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry


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