In vitro toxicity of topical antimicrobial agents to human fibroblasts

Robert L. McCauley; Hugo A. Linares; Virginia Pelligrini; David N. Herndon; Martin C. Robson; John P. Heggers

doi:10.1016/0022-4804(89)90069-3

In vitro toxicity of topical antimicrobial agents to human fibroblasts

Robert L. McCauley, Hugo A. Linares, Virginia Pelligrini, David N. Herndon, Martin C. Robson, John P. Heggers

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.

Original language	English (US)
Pages (from-to)	267-274
Number of pages	8
Journal	Journal of Surgical Research
Volume	46
Issue number	3
DOIs	https://doi.org/10.1016/0022-4804(89)90069-3
State	Published - Mar 1989
Externally published	Yes

ASJC Scopus subject areas

Surgery

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10.1016/0022-4804(89)90069-3

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title = "In vitro toxicity of topical antimicrobial agents to human fibroblasts",

abstract = "Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.",

author = "McCauley, {Robert L.} and Linares, {Hugo A.} and Virginia Pelligrini and Herndon, {David N.} and Robson, {Martin C.} and Heggers, {John P.}",

year = "1989",

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AU - McCauley, Robert L.

AU - Linares, Hugo A.

AU - Pelligrini, Virginia

AU - Herndon, David N.

AU - Robson, Martin C.

AU - Heggers, John P.

PY - 1989/3

Y1 - 1989/3

N2 - Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.

AB - Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.

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ER -

In vitro toxicity of topical antimicrobial agents to human fibroblasts

Abstract

ASJC Scopus subject areas

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