In vitro antibacterial and in vivo brine shrimp lethal active compounds isolated from the leaves of Saurauia roxburghii

Seven triterpenoids namely, ursolic acid (1) cis-3-O-p-hydroxycinnamoyl ursolic acid (2), trans-3-O-p-hydroxycinnamoyl ursolic acid (3), oleanolic acid (4), corosolic acid (5), maslinic acid (6) and β-amyrin (7) were isolated from the leaves of Saurauia roxburghii based on spectroscopic indication. Compounds 2, 3 and 4 were isolated from this plant for the first time. The LC50 for crude ethanol, n-hexane, chloroform, ethyl-acetate; column fractions H-13 of the crude n-hexane, C-19 of the crude chloroform and three pure compounds 1, 3 and 4 were found to be 12.59, 14.79, 14.06, 11.75, 10.96, 4.37, 6.92 and 11.88 μg mLG1, respectively. The crude ethanol extract exhibited good; n-hexane, chloroform, ethyl-acetate extracts exhibited moderate; five column fractions (H-13, C-15, C-19, E-18 and E-24) exhibited low to mild and three pure compounds (1, 3 and 4) presented moderate antibacterial activity against maximum of the tested bacteria.