TY - JOUR
T1 - In situ expression of interleukin-10 and interleukin-12 in active human cutaneous leishmaniasis
AU - Melby, Peter C.
AU - Andrade-Narvaez, Fernando
AU - Darnell, Barbara J.
AU - Valencia-Pacheco, Guillermo
N1 - Funding Information:
This work was supported by the National Insti-tuteso f Health (AI-31065) and was presentedin part at the International Congress of Parasitology, Izmir, Turkey, October 1994 (Abstract 549). The study was approvedb y the Human ResearchS ubjects Committees at the University of Texas Health Sciences Center at San Antonio, and the Universidad Au-tonoma de Yucatan, Merida, Mexico.
PY - 1996/9
Y1 - 1996/9
N2 - Th1-type cellular immune responses (interferon-γ) play a critical role in protection against Leishmania spp. infection, whereas Th2-type cytokines (interleukin (IL)-4, IL-10) have a counter-protective effect. IL-12, a potent inducer of Th1-type cellular immune responses, may play a pivotal role in the development of a protective response. We found that IL-10 and IL-12 mRNAs were expressed in most lesions of individuals with active cutaneous leishmaniasis. The quantity of IL-12 mRNA was highly variable but correlated strongly with the level of interferon-γ expression. IL-12 expression also paralleled the expression of IL-10, a potent in vitro suppressor of IL-12 and interferon-γ production. The more chronic, non-healing lesions generally had higher levels of IL-12 mRNA indicating that the expression of this cytokine alone was not sufficient to induce healing. Although the in situ production of IL-10 did not appear to block IL-12 expression, IL-10 may still promote disease by direct suppression of macrophage activation.
AB - Th1-type cellular immune responses (interferon-γ) play a critical role in protection against Leishmania spp. infection, whereas Th2-type cytokines (interleukin (IL)-4, IL-10) have a counter-protective effect. IL-12, a potent inducer of Th1-type cellular immune responses, may play a pivotal role in the development of a protective response. We found that IL-10 and IL-12 mRNAs were expressed in most lesions of individuals with active cutaneous leishmaniasis. The quantity of IL-12 mRNA was highly variable but correlated strongly with the level of interferon-γ expression. IL-12 expression also paralleled the expression of IL-10, a potent in vitro suppressor of IL-12 and interferon-γ production. The more chronic, non-healing lesions generally had higher levels of IL-12 mRNA indicating that the expression of this cytokine alone was not sufficient to induce healing. Although the in situ production of IL-10 did not appear to block IL-12 expression, IL-10 may still promote disease by direct suppression of macrophage activation.
KW - Cellular immunity
KW - Cytokine
KW - Interleukin-10
KW - Interleukin-12
KW - Leishmania
KW - Polymerase chain reaction
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U2 - 10.1016/0928-8244(96)00047-8
DO - 10.1016/0928-8244(96)00047-8
M3 - Article
C2 - 8880134
AN - SCOPUS:0030248330
SN - 0928-8244
VL - 15
SP - 101
EP - 107
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 2-3
ER -