Impaired nonspecific cellular immunity in experimental cholestasis

P. T. Roughneen; D. B. Drath; A. D. Kulkarni; B. J. Rowlands

doi:10.1097/00000658-198711000-00004

Impaired nonspecific cellular immunity in experimental cholestasis

P. T. Roughneen, D. B. Drath, A. D. Kulkarni, B. J. Rowlands

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Abstract

The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C(5a) and FMLP, phagocytosis of ¹⁴C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p < 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of ¹⁴C labeled Staphylococcus aureus was depressed in BDL (p < 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p < 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice.

Original language	English (US)
Pages (from-to)	578-582
Number of pages	5
Journal	Annals of surgery
Volume	206
Issue number	5
DOIs	https://doi.org/10.1097/00000658-198711000-00004
State	Published - 1987
Externally published	Yes

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Surgery

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title = "Impaired nonspecific cellular immunity in experimental cholestasis",

abstract = "The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C(5a) and FMLP, phagocytosis of 14C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p < 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of 14C labeled Staphylococcus aureus was depressed in BDL (p < 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p < 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice.",

author = "Roughneen, {P. T.} and Drath, {D. B.} and Kulkarni, {A. D.} and Rowlands, {B. J.}",

year = "1987",

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T1 - Impaired nonspecific cellular immunity in experimental cholestasis

AU - Roughneen, P. T.

AU - Drath, D. B.

AU - Kulkarni, A. D.

AU - Rowlands, B. J.

PY - 1987

Y1 - 1987

N2 - The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C(5a) and FMLP, phagocytosis of 14C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p < 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of 14C labeled Staphylococcus aureus was depressed in BDL (p < 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p < 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice.

AB - The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C(5a) and FMLP, phagocytosis of 14C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p < 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of 14C labeled Staphylococcus aureus was depressed in BDL (p < 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p < 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice.

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JO - Annals of surgery

JF - Annals of surgery

IS - 5

ER -

Impaired nonspecific cellular immunity in experimental cholestasis

Abstract

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