Immunohistochemical localization of 5-HT(2A) receptors in peripheral sensory axons in rat glabrous skin

Susan M. Carlton; Richard E. Coggeshall

doi:10.1016/S0006-8993(97)00489-7

Immunohistochemical localization of 5-HT(2A) receptors in peripheral sensory axons in rat glabrous skin

Susan M. Carlton, Richard E. Coggeshall

Neuroscience & Cell

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Serotonin (5-hydroxytryptamine, 5-HT) is a well known inflammatory mediator and algesic substance. It has been hypothesized that 5-HT can have a direct action on peripheral sensory axons, but there has been no anatomical demonstration of 5-HT receptors on peripheral primary afferent processes. The present study shows that 32% of unmyelinated axons at the dermal-epidermal junction are immunohistochemically stained with antibodies directed against the 5-HT(2A) receptor providing anatomical evidence that 5-HT can have a direct effect on sensory fibers in the skin. Furthermore, encapsulated nerve endings in Pacinian corpuscles also contain reaction product following immunostaining for 5-HT(2A) receptors, indicating that large myelinated axons can be activated by endogenous serotonin. These data suggest that peripherally acting 5-HT(2A) antagonists may be effective in reducing pain of peripheral origin.

Original language	English (US)
Pages (from-to)	271-275
Number of pages	5
Journal	Brain Research
Volume	763
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(97)00489-7
State	Published - Jul 25 1997

Keywords

Catecholamine
Pain
Peripheral sensitization
Ultrastructure

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/S0006-8993(97)00489-7

Cite this

@article{94acb5b18e15410896b29b953280dbaf,

title = "Immunohistochemical localization of 5-HT(2A) receptors in peripheral sensory axons in rat glabrous skin",

abstract = "Serotonin (5-hydroxytryptamine, 5-HT) is a well known inflammatory mediator and algesic substance. It has been hypothesized that 5-HT can have a direct action on peripheral sensory axons, but there has been no anatomical demonstration of 5-HT receptors on peripheral primary afferent processes. The present study shows that 32% of unmyelinated axons at the dermal-epidermal junction are immunohistochemically stained with antibodies directed against the 5-HT(2A) receptor providing anatomical evidence that 5-HT can have a direct effect on sensory fibers in the skin. Furthermore, encapsulated nerve endings in Pacinian corpuscles also contain reaction product following immunostaining for 5-HT(2A) receptors, indicating that large myelinated axons can be activated by endogenous serotonin. These data suggest that peripherally acting 5-HT(2A) antagonists may be effective in reducing pain of peripheral origin.",

keywords = "Catecholamine, Pain, Peripheral sensitization, Ultrastructure",

author = "Carlton, {Susan M.} and Coggeshall, {Richard E.}",

note = "Funding Information: The authors thank Brenda Kenworthy for her excellent secretarial assistance and Zhixia Ding for assistance in the immunohistochemistry and electron microscopy. This work was supported by NIH NS11255 to S.M.C. and R.E.C., NS27910 to S.M.C and NS10161 to R.E.C. ",

year = "1997",

month = jul,

day = "25",

doi = "10.1016/S0006-8993(97)00489-7",

language = "English (US)",

volume = "763",

pages = "271--275",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Immunohistochemical localization of 5-HT(2A) receptors in peripheral sensory axons in rat glabrous skin

AU - Carlton, Susan M.

AU - Coggeshall, Richard E.

N1 - Funding Information: The authors thank Brenda Kenworthy for her excellent secretarial assistance and Zhixia Ding for assistance in the immunohistochemistry and electron microscopy. This work was supported by NIH NS11255 to S.M.C. and R.E.C., NS27910 to S.M.C and NS10161 to R.E.C.

PY - 1997/7/25

Y1 - 1997/7/25

N2 - Serotonin (5-hydroxytryptamine, 5-HT) is a well known inflammatory mediator and algesic substance. It has been hypothesized that 5-HT can have a direct action on peripheral sensory axons, but there has been no anatomical demonstration of 5-HT receptors on peripheral primary afferent processes. The present study shows that 32% of unmyelinated axons at the dermal-epidermal junction are immunohistochemically stained with antibodies directed against the 5-HT(2A) receptor providing anatomical evidence that 5-HT can have a direct effect on sensory fibers in the skin. Furthermore, encapsulated nerve endings in Pacinian corpuscles also contain reaction product following immunostaining for 5-HT(2A) receptors, indicating that large myelinated axons can be activated by endogenous serotonin. These data suggest that peripherally acting 5-HT(2A) antagonists may be effective in reducing pain of peripheral origin.

AB - Serotonin (5-hydroxytryptamine, 5-HT) is a well known inflammatory mediator and algesic substance. It has been hypothesized that 5-HT can have a direct action on peripheral sensory axons, but there has been no anatomical demonstration of 5-HT receptors on peripheral primary afferent processes. The present study shows that 32% of unmyelinated axons at the dermal-epidermal junction are immunohistochemically stained with antibodies directed against the 5-HT(2A) receptor providing anatomical evidence that 5-HT can have a direct effect on sensory fibers in the skin. Furthermore, encapsulated nerve endings in Pacinian corpuscles also contain reaction product following immunostaining for 5-HT(2A) receptors, indicating that large myelinated axons can be activated by endogenous serotonin. These data suggest that peripherally acting 5-HT(2A) antagonists may be effective in reducing pain of peripheral origin.

KW - Catecholamine

KW - Pain

KW - Peripheral sensitization

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0030790011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030790011&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(97)00489-7

DO - 10.1016/S0006-8993(97)00489-7

M3 - Article

C2 - 9296571

AN - SCOPUS:0030790011

SN - 0006-8993

VL - 763

SP - 271

EP - 275

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Immunohistochemical localization of 5-HT(2A) receptors in peripheral sensory axons in rat glabrous skin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this