Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

T. Ikegami; M. Saijo; M. Niikura; M. E. Miranda; A. B. Calaor; M. Hernandez; D. L. Manalo; I. Kurane; Y. Yoshikawa; S. Morikawa

doi:10.1017/s0950268803008264

Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

T. Ikegami, M. Saijo, M. Niikura, M. E. Miranda, A. B. Calaor, M. Hernandez, D. L. Manalo, I. Kurane, Y. Yoshikawa, S. Morikawa

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17 Scopus citations

Abstract

We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360-739), and RΔ6 (aa 451-551) and/or RΔ8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

Original language	English (US)
Pages (from-to)	533-539
Number of pages	7
Journal	Epidemiology and infection
Volume	130
Issue number	3
DOIs	https://doi.org/10.1017/s0950268803008264
State	Published - Jun 2003
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Infectious Diseases

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title = "Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus",

abstract = "We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360-739), and RΔ6 (aa 451-551) and/or RΔ8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.",

author = "T. Ikegami and M. Saijo and M. Niikura and Miranda, {M. E.} and Calaor, {A. B.} and M. Hernandez and Manalo, {D. L.} and I. Kurane and Y. Yoshikawa and S. Morikawa",

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language = "English (US)",

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AU - Ikegami, T.

AU - Saijo, M.

AU - Niikura, M.

AU - Miranda, M. E.

AU - Calaor, A. B.

AU - Hernandez, M.

AU - Manalo, D. L.

AU - Kurane, I.

AU - Yoshikawa, Y.

AU - Morikawa, S.

PY - 2003/6

Y1 - 2003/6

N2 - We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360-739), and RΔ6 (aa 451-551) and/or RΔ8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

AB - We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360-739), and RΔ6 (aa 451-551) and/or RΔ8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

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Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

Abstract

ASJC Scopus subject areas

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