Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies

B. K. Sil; M. R. Wills; J. X. Cao; R. Sharda; M. A. Islam; D. J. Stagg; A. D. Jennings; C. A. Gibson; A. D.T. Barrett

doi:10.1016/0264-410X(92)90372-Q

Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies

B. K. Sil, M. R. Wills, J. X. Cao, R. Sharda, M. A. Islam, D. J. Stagg, A. D. Jennings, C. A. Gibson, A. D.T. Barrett

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibition activity while only a minority (24%) elicited neutralization. None of the mAbs prepared against SA14-5-3, parent of SA14-14-2, elicited neutralization while the only mAbs prepared against SA14-14-2 that elicited neutralization recognized flavivirus cross-reactive epitopes. In comparison, mAbs raised against wild-type strains showed that a spectrum of epitopes with different specificities, including JE type-specific epitopes, elicited neutralizing activity. Two mAbs, prepared against SA14-5-3 virus, were found to be vaccine-specific and five, prepared against strains SA14 and G8924, were wild-type-specific.

Original language	English (US)
Pages (from-to)	329-333
Number of pages	5
Journal	Vaccine
Volume	10
Issue number	5
DOIs	https://doi.org/10.1016/0264-410X(92)90372-Q
State	Published - 1992
Externally published	Yes

Keywords

Japanese encephalitis
flavivirus
monoclonal antibodies
vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0264-410X(92)90372-Q

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Sil, B. K., Wills, M. R., Cao, J. X., Sharda, R., Islam, M. A., Stagg, D. J., Jennings, A. D., Gibson, C. A., & Barrett, A. D. T. (1992). Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies. Vaccine, 10(5), 329-333. https://doi.org/10.1016/0264-410X(92)90372-Q

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title = "Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies",

abstract = "A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibition activity while only a minority (24%) elicited neutralization. None of the mAbs prepared against SA14-5-3, parent of SA14-14-2, elicited neutralization while the only mAbs prepared against SA14-14-2 that elicited neutralization recognized flavivirus cross-reactive epitopes. In comparison, mAbs raised against wild-type strains showed that a spectrum of epitopes with different specificities, including JE type-specific epitopes, elicited neutralizing activity. Two mAbs, prepared against SA14-5-3 virus, were found to be vaccine-specific and five, prepared against strains SA14 and G8924, were wild-type-specific.",

keywords = "Japanese encephalitis, flavivirus, monoclonal antibodies, vaccine",

author = "Sil, {B. K.} and Wills, {M. R.} and Cao, {J. X.} and R. Sharda and Islam, {M. A.} and Stagg, {D. J.} and Jennings, {A. D.} and Gibson, {C. A.} and Barrett, {A. D.T.}",

note = "Funding Information: This work was funded in part by a grant from the Vaccine Development Programme of the World Health Organization. B.K.S. was supported by the Association of Commonwealth Universities, J.X.C. by the British Council/Chinese government, M.A.I. by the British Council and R.S. by the Rotary Foundation. The authors thank Dennis Trent (CDC, Fort Collins) for providing strains of JE virus. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

doi = "10.1016/0264-410X(92)90372-Q",

language = "English (US)",

volume = "10",

pages = "329--333",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Ltd",

number = "5",

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TY - JOUR

T1 - Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies

AU - Sil, B. K.

AU - Wills, M. R.

AU - Cao, J. X.

AU - Sharda, R.

AU - Islam, M. A.

AU - Stagg, D. J.

AU - Jennings, A. D.

AU - Gibson, C. A.

AU - Barrett, A. D.T.

N1 - Funding Information: This work was funded in part by a grant from the Vaccine Development Programme of the World Health Organization. B.K.S. was supported by the Association of Commonwealth Universities, J.X.C. by the British Council/Chinese government, M.A.I. by the British Council and R.S. by the Rotary Foundation. The authors thank Dennis Trent (CDC, Fort Collins) for providing strains of JE virus. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1992

Y1 - 1992

N2 - A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibition activity while only a minority (24%) elicited neutralization. None of the mAbs prepared against SA14-5-3, parent of SA14-14-2, elicited neutralization while the only mAbs prepared against SA14-14-2 that elicited neutralization recognized flavivirus cross-reactive epitopes. In comparison, mAbs raised against wild-type strains showed that a spectrum of epitopes with different specificities, including JE type-specific epitopes, elicited neutralizing activity. Two mAbs, prepared against SA14-5-3 virus, were found to be vaccine-specific and five, prepared against strains SA14 and G8924, were wild-type-specific.

AB - A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibition activity while only a minority (24%) elicited neutralization. None of the mAbs prepared against SA14-5-3, parent of SA14-14-2, elicited neutralization while the only mAbs prepared against SA14-14-2 that elicited neutralization recognized flavivirus cross-reactive epitopes. In comparison, mAbs raised against wild-type strains showed that a spectrum of epitopes with different specificities, including JE type-specific epitopes, elicited neutralizing activity. Two mAbs, prepared against SA14-5-3 virus, were found to be vaccine-specific and five, prepared against strains SA14 and G8924, were wild-type-specific.

KW - Japanese encephalitis

KW - flavivirus

KW - monoclonal antibodies

KW - vaccine

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JO - Vaccine

JF - Vaccine

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ER -

Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies

Abstract

Keywords

ASJC Scopus subject areas

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