Immunization of mice against west nile virus with recombinant envelope protein

T. Wang, J. F. Anderson, L. A. Magnarelli, S. J. Wong, R. A. Koski, E. Fikrig

Research output: Contribution to journalArticlepeer-review

86 Scopus citations


West Nile (WN) virus is a mosquito-borne flavivirus that emerged in the United States in 1999 and can cause fatal encephalitis. Envelope (E) protein cDNA from a WN virus isolate recovered from Culex pipiens in Connecticut was expressed in Escherichia coli. The recombinant E protein was purified and used as Ag in immunoblot assays and immunization experiments. Patients with WN virus infection had Abs that recognized the recombinant E protein. C3H/HeN mice immunized with E protein developed E protein Abs and were protected from infection with WN virus. Passive administration of E protein antisera was also sufficient to afford immunity. E protein is a candidate vaccine to prevent WN virus infection.

Original languageEnglish (US)
Pages (from-to)5273-5277
Number of pages5
JournalJournal of Immunology
Issue number9
StatePublished - Nov 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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