Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates

Gary Wong; Jason S. Richardson; Stéphane Pillet; Ami Patel; Xiangguo Qiu; Judie Alimonti; Jeff Hogan; Yi Zhang; Ayato Takada; Heinz Feldmann; Gary P. Kobinger

doi:10.1126/scitranslmed.3004582

Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates

Gary Wong, Jason S. Richardson, Stéphane Pillet, Ami Patel, Xiangguo Qiu, Judie Alimonti, Jeff Hogan, Yi Zhang, Ayato Takada, Heinz Feldmann, Gary P. Kobinger

Research output: Contribution to journal › Article › peer-review

Abstract

Ebola virus causes severe hemorrhagic fever in susceptible hosts. Currently, no licensed vaccines or treatments are available; however, several experimental vaccines have been successful in protecting rodents and nonhuman primates (NHPs) from the lethal Zaire ebolavirus (ZEBOV) infection. The objective of this study was to evaluate immune responses correlating with survival in these animals after lethal challenge with ZEBOV. Knockout mice with impaired ability to generate normal T and/or B cell responses were vaccinated and challenged with ZEBOV. Vaccine-induced protection in mice was mainly mediated by B cells and CD4+ T cells. Vaccinated, outbred guinea pigs and NHPs demonstrated the highest correlation between survival and levels of total immunoglobulin G (IgG) specific to the ZEBOV glycoprotein (ZGP). These results highlight the relevance of total ZGP-specific IgG levels as a meaningful correlate of protection against ZEBOV exposure.

Original language	English (US)
Article number	158ra146
Journal	Science Translational Medicine
Volume	4
Issue number	158
DOIs	https://doi.org/10.1126/scitranslmed.3004582
State	Published - Oct 31 2012
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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title = "Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates",

abstract = "Ebola virus causes severe hemorrhagic fever in susceptible hosts. Currently, no licensed vaccines or treatments are available; however, several experimental vaccines have been successful in protecting rodents and nonhuman primates (NHPs) from the lethal Zaire ebolavirus (ZEBOV) infection. The objective of this study was to evaluate immune responses correlating with survival in these animals after lethal challenge with ZEBOV. Knockout mice with impaired ability to generate normal T and/or B cell responses were vaccinated and challenged with ZEBOV. Vaccine-induced protection in mice was mainly mediated by B cells and CD4+ T cells. Vaccinated, outbred guinea pigs and NHPs demonstrated the highest correlation between survival and levels of total immunoglobulin G (IgG) specific to the ZEBOV glycoprotein (ZGP). These results highlight the relevance of total ZGP-specific IgG levels as a meaningful correlate of protection against ZEBOV exposure.",

author = "Gary Wong and Richardson, {Jason S.} and St{\'e}phane Pillet and Ami Patel and Xiangguo Qiu and Judie Alimonti and Jeff Hogan and Yi Zhang and Ayato Takada and Heinz Feldmann and Kobinger, {Gary P.}",

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language = "English (US)",

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AU - Wong, Gary

AU - Richardson, Jason S.

AU - Pillet, Stéphane

AU - Patel, Ami

AU - Qiu, Xiangguo

AU - Alimonti, Judie

AU - Hogan, Jeff

AU - Zhang, Yi

AU - Takada, Ayato

AU - Feldmann, Heinz

AU - Kobinger, Gary P.

PY - 2012/10/31

Y1 - 2012/10/31

N2 - Ebola virus causes severe hemorrhagic fever in susceptible hosts. Currently, no licensed vaccines or treatments are available; however, several experimental vaccines have been successful in protecting rodents and nonhuman primates (NHPs) from the lethal Zaire ebolavirus (ZEBOV) infection. The objective of this study was to evaluate immune responses correlating with survival in these animals after lethal challenge with ZEBOV. Knockout mice with impaired ability to generate normal T and/or B cell responses were vaccinated and challenged with ZEBOV. Vaccine-induced protection in mice was mainly mediated by B cells and CD4+ T cells. Vaccinated, outbred guinea pigs and NHPs demonstrated the highest correlation between survival and levels of total immunoglobulin G (IgG) specific to the ZEBOV glycoprotein (ZGP). These results highlight the relevance of total ZGP-specific IgG levels as a meaningful correlate of protection against ZEBOV exposure.

AB - Ebola virus causes severe hemorrhagic fever in susceptible hosts. Currently, no licensed vaccines or treatments are available; however, several experimental vaccines have been successful in protecting rodents and nonhuman primates (NHPs) from the lethal Zaire ebolavirus (ZEBOV) infection. The objective of this study was to evaluate immune responses correlating with survival in these animals after lethal challenge with ZEBOV. Knockout mice with impaired ability to generate normal T and/or B cell responses were vaccinated and challenged with ZEBOV. Vaccine-induced protection in mice was mainly mediated by B cells and CD4+ T cells. Vaccinated, outbred guinea pigs and NHPs demonstrated the highest correlation between survival and levels of total immunoglobulin G (IgG) specific to the ZEBOV glycoprotein (ZGP). These results highlight the relevance of total ZGP-specific IgG levels as a meaningful correlate of protection against ZEBOV exposure.

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Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates

Abstract

ASJC Scopus subject areas

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