IL-10 modulates placental responses to TLR ligands

Mehmet Bayraktar, Morgan Peltier, Anna Vetrano, Yuko Arita, Ellen Gurzenda, Ansamma Joseph, Jeffrey Kazzaz, Surendra Sharma, Nazeeh Hanna

Research output: Contribution to journalArticlepeer-review


Problem: Intra-uterine infections increase production of pro-inflammatory cytokines. It is unclear whether different infectious agents determine the relative expression of pro-and anti-inflammatory cytokines. Methods of study: We compared the placental inflammatory response induced by bacterial lipopolysaccharide (LPS, endotoxin from Gram-negative bacteria) with those induced by lipoteichoic acid (LTA, a cell wall component of Gram-positive bacteria). Placental explants from term delivery were treated with either LPS or LTA, in the presence or absence of IL-10, for 24 hrs. Cytokines, prostaglandin E2 (PGE2) production and cyclo-oxygenase-2 (COX-2) expression were quantified. Results: Both LTA and LPS significantly induced several cytokines with LPS eliciting more potent effects. IL-6 and IL-8 were induced to comparable levels in response to both LTA and LPS whereas monocyte chemotactic protein-1 (MCP-1) production was induced more by LTA, demonstrating a differential placental response to a specific toll-like receptor (TLR) ligand. IL-10 treatment significantly reduced most pro-inflammatory cytokines as well as PGE2 induced by both LPS and LTA. Interestingly, IL-10 down-regulated LTA-mediated MCP1 induction, but not that mediated by LPS. Moreover, IL-10 was more effective in down-regulating PGE2 after LPS- when compared with LTA stimulation. Conclusions: Our results demonstrate that placental exposure to LTA and LPS appear to trigger distinct cytokine responses that can be modulated by IL-10.

Original languageEnglish (US)
Pages (from-to)390-399
Number of pages10
JournalAmerican Journal of Reproductive Immunology
Issue number6
StatePublished - Dec 2009
Externally publishedYes


  • IL-10
  • Inflammation
  • Lipopolysaccharide
  • Lipoteichoic acid
  • Pre-term labor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology


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