@article{48cbdda182eb41ad90a72313ad90a0f6,
title = "Identification of tick periviscerokinin, the first neurohormone of Ixodidae: Single cell analysis by means of MALDI-TOF/TOF mass spectrometry",
abstract = "The first peptidergic neurohormone from the ticks Ixodes ricinus and Boophilus microplus has been identified by using a combination of immunocytochemistry and mass spectrometric analysis of single cells. The novel peptide (Ixori-PVK, PALIPFPRV-NH2) shows a high sequence homology with members of the insect periviscerokinin/CAP2b peptides that in insects are involved in the regulation of water balance. The function of this peptide in ticks is still unknown, but these pests consume large amounts of blood in a single blood meal which is a challenge for the regulation of diuretic processes. Thus, the novel peptide may be involved in one of the key physiological processes in ticks. High energy collision-induced dissociation was successfully used to distinguish between Leu/Ile ambiguities in single cell preparations. This is the first successful de novo sequencing of a peptide from single cell preparations of arthropods.",
keywords = "Boophilus, Ixodes, MALDI-TOF/TOF mass spectrometry, Neuropeptide, Periviscerokinin, Single cell analysis, Tick",
author = "Susanne Neupert and Reinhard Predel and Russell, {William K.} and Ronald Davies and Pietrantonio, {Patricia V.} and Nachman, {Ronald J.}",
note = "Funding Information: The authors thank Renate Winkler and Sabine Kaltofen (Jena, Germany) for providing the European hard tick I. ricinus L., Manfred Eckert (Jena, Germany) for the PVK antiserum, Dick R. N{\"a}ssel (Stockholm, Sweden) for the leukokinin antiserum, and Allison Strey for technical assistance (College Station, TX). This study was supported by a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1), a Binational Agricultural Research and Development Grant (BARD #IS-3356-02) (R.J.N.), a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (R.J.N., G.M.C.), grant from the USDA/DOD DWFP Initiative (#0500-32000-001-01R) (R.J.N.), a grants provided to the Laboratory for Biological Mass Spectrometry at Texas A&M University, College Station: R01 RR019587 NIH and DE-FG02-04ER15520 and by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (CSREES), and Grant NRI/CSREES 2003-01347 (P.V.P.). ",
year = "2005",
month = dec,
day = "30",
doi = "10.1016/j.bbrc.2005.10.165",
language = "English (US)",
volume = "338",
pages = "1860--1864",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",
}